Schall R F, Fraser A S, Hansen H W, Kern C W, Tenoso H J
Clin Chem. 1978 Oct;24(10):1801-4.
We describe an immunoassay for thyroxine in serum. In the assay specific antibody covalently bonded to latex particles is used, along with horseradish peroxidase as the label, and o-phenylenediamine as the chromogen. The flexible protocol is designed for manual execution. Performance is similar to that of the highest-sensitivity thyroxine radioimmunoassays. Results correlate well with radioimmunoassay (r = 0.99, slope = 0.93, y-intercept = 2.4 microgram/liter for 201 samples) and an automated enzyme immunoassay (r = 0.97, slope = 0.99, y-intercept = 4.7 coefficients of variation are less than 7.2% over the entire useful range of the assay (20--240 microgram/liter). The limit of detection is less than 94 pg/tube at 20 microgram/liter. Only D-thyroxine is known to interfere with serum assays. This assay has no discernible protein effect from 40 to 80 g of protein per liter, unlike many thyroxine radioimmunoassays. Serum preservatives known to be peroxidase inhibitors do not adversely affect assay performance because of the 56-fold dilution in the final assay mixture. Hemolyzed serum and EDTA-treated plasmas are unsuitable for this assay.
我们描述了一种血清甲状腺素免疫测定法。在该测定法中，使用与乳胶颗粒共价结合的特异性抗体，同时以辣根过氧化物酶作为标记物，邻苯二胺作为显色剂。灵活的方案设计用于手动操作。其性能与最高灵敏度的甲状腺素放射免疫测定法相似。结果与放射免疫测定法（201个样本，r = 0.99，斜率 = 0.93，截距 = 2.4微克/升）和自动酶免疫测定法（r = 0.97，斜率 = 0.99，截距 = 4.7）相关性良好，在测定的整个有效范围内（20 - 240微克/升）变异系数小于7.2%。在20微克/升时检测限小于94皮克/管。已知只有D - 甲状腺素会干扰血清测定。与许多甲状腺素放射免疫测定法不同，该测定法在每升40至80克蛋白质范围内没有明显的蛋白质效应。已知的过氧化物酶抑制剂血清防腐剂不会对测定性能产生不利影响，因为在最终测定混合物中稀释了56倍。溶血血清和经乙二胺四乙酸处理的血浆不适用于此测定法。
