Lokki M L, Koskimies S A
FRC Blood Transfusion Service, Helsinki, Finland.
Immunogenetics. 1991;34(4):242-6. doi: 10.1007/BF00215259.
After separating the *F and *S alleles by electrophoresis the allele-specific hemolytic activity was detected by agarose overlay method using the programmable densitometer for scanning. The hemolytic activity of BF allotypes was analyzed from 81 individuals. In thirteen FS heterozygous serum samples BF F had lower hemolysis than BF S. Four FF homozygous samples also exhibited lower hemolysis than a homozygous control sample. The low hemolytic activity of F in FS heterozygotes was not due to decreased protein concentrations relative to S. On the contrary, BF F was associated with higher protein concentration than BF S. The relative quantitation of the allele specific BF protein was done by crossed immunoelectrophoresis. BF F with low hemolytic activity but with high protein concentration associated strongly with HLA B35 phenotype and the family material confirmed the association with the haplotypes A3, Cw4, B35, DR1, BFFB, C4A3BQO (or A2BQO, A3,2BQO). The results suggest that particular MHC haplotypes contain a factor B allele with encoding for poor hemolytic activity or that MHC haplotype specific regulatory elements affect pre- or post-translational activity levels.
通过电泳分离F和S等位基因后，使用可编程密度计扫描的琼脂糖覆盖法检测等位基因特异性溶血活性。分析了81名个体的BF别型的溶血活性。在13份FS杂合血清样本中，BF F的溶血低于BF S。4份FF纯合样本的溶血也低于一份纯合对照样本。FS杂合子中F的低溶血活性并非由于相对于S的蛋白质浓度降低。相反，BF F与比BF S更高的蛋白质浓度相关。通过交叉免疫电泳对等位基因特异性BF蛋白进行相对定量。具有低溶血活性但高蛋白浓度的BF F与HLA B35表型密切相关，家族资料证实其与单倍型A3、Cw4、B35、DR1、BFFB、C4A3BQO（或A2BQO、A3,2BQO）相关。结果表明，特定的MHC单倍型包含一个编码溶血活性差的B因子等位基因，或者MHC单倍型特异性调节元件影响翻译前或翻译后的活性水平。
