Bodinier M, Leroy M, Ah-Leung S, Blanc F, Tranquet O, Denery-Papini S, Wal J-M, Adel-Patient K
INRA, UR1268, Unite de recherche sur les Biopolymeres, leurs Interactions et Assemblages (BIA), F-44316 Nantes, France.
J Agric Food Chem. 2009 Feb 25;57(4):1219-25. doi: 10.1021/jf802898u.
We developed a mouse model of allergy to wheat flour gliadins, a protein fraction containing major wheat allergens. We compared the antibody responses (i.e., specific IgE and IgG1) and the profiles of cytokines secreted by reactivated splenocytes induced after intraperitoneal injections of gliadins in three strains of mice, namely, Balb/cJ, B10.A, and C3H/HeJ. The intensities of the allergic reactions elicited by intranasal challenge were also compared. Both the sensitization and elicitation were the highest in Balb/cJ mice, whereas weak or no reaction was observed in the others strains. Interestingly, the specificity of the mouse IgE against the different gliadins (i.e., alpha-, beta-, gamma-, omega 1,2-, and omega 5-gliadin) was similar to that observed in children allergic to wheat flour. Balb/cJ mice may thus provide a relevant model for the study of sensitization and elicitation by wheat gliadins and for improving our understanding of the specific role and mechanisms of action of the different classes of gliadins.
我们构建了一种对小麦粉麦醇溶蛋白过敏的小鼠模型，麦醇溶蛋白是一种包含主要小麦过敏原的蛋白质组分。我们比较了在Balb/cJ、B10.A和C3H/HeJ这三种小鼠品系中，腹腔注射麦醇溶蛋白后重新激活的脾细胞分泌的抗体反应（即特异性IgE和IgG1）以及细胞因子谱。还比较了鼻内激发引起的过敏反应强度。致敏和激发在Balb/cJ小鼠中最为强烈，而在其他品系中观察到的反应较弱或无反应。有趣的是，小鼠IgE针对不同麦醇溶蛋白（即α-、β-、γ-、ω1,2-和ω5-麦醇溶蛋白）的特异性与对小麦粉过敏儿童中观察到的相似。因此，Balb/cJ小鼠可能为研究小麦麦醇溶蛋白的致敏和激发以及增进我们对不同类别麦醇溶蛋白的具体作用和作用机制的理解提供一个相关模型。
