“无过氧化氢酶血症样”红细胞与过氧化氢的体外研究：关注溶血抗性红细胞的形成。
In vitro studies with 'acatalasemic-like' erythrocytes and hydrogen peroxide: attention to the formation of lysis resistant erythrocytes.
机构信息
Serviço Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.
出版信息
Int J Lab Hematol. 2010 Feb;32(1 Pt 2):127-31. doi: 10.1111/j.1751-553X.2008.01129.x. Epub 2009 Jan 17.
Abstract
Hemolysis assays, using hydrogen peroxide as an oxidant injury agent and sodium azide as a catalase inhibitor, were performed to evaluate how assay conditions affect the development of lysis resistant erythrocytes. The concentration of hydrogen peroxide should be carefully selected for use in hemolysis assays, as it was found to affect the number of water resistant erythrocytes.
摘要
溶血实验中，使用过氧化氢作为氧化剂损伤剂，叠氮化钠作为过氧化氢酶抑制剂，以评估检测条件如何影响抗溶血红细胞的发展。应仔细选择用于溶血实验的过氧化氢浓度，因为发现其会影响抗水红细胞的数量。
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