Masuoka N, Wakimoto M, Ubuka T, Nakano T
Department of Biochemistry, Okayama University Medical School, Japan.
Clin Chim Acta. 1996 Oct 29;254(2):101-12. doi: 10.1016/0009-8981(96)06374-7.
A new method of hydrogen peroxide determination for the measurement of catalase activity and rates of hydrogen peroxide removal by erythrocytes was described. Hydrogen peroxide was determined by converting it to the indamine dye with a water-soluble ironporphyrin and measuring the absorbance at 590 nm. This method was applied to the assay of catalase in hemolysates from human, rat and mouse blood. The activities obtained were in agreement with those obtained by other methods including UV method. The present method was also applied to the determination of rates of hydrogen peroxide removal by intact erythrocytes from human subjects, rats and mice. Data suggested that normal erythrocytes have substantial capacity to remove extracellular hydrogen peroxide. From the measurement of catalase activity in erythrocytes treated with 3-amino-1,2,4-triazole and rates of hydrogen peroxide removal by the erythrocytes, it is deduced that rate constants related to the hemoglobin content (k/g Hb) for hydrogen peroxide removal by catalase in normal and acatalasemic erythrocytes are 42.0 +/- 6.0 and 8.0 +/- 3.0, respectively.
描述了一种用于测定过氧化氢酶活性以及红细胞清除过氧化氢速率的过氧化氢测定新方法。通过使用水溶性铁卟啉将过氧化氢转化为吲哚胺染料并在590nm处测量吸光度来测定过氧化氢。该方法应用于人、大鼠和小鼠血液溶血产物中过氧化氢酶的测定。获得的活性与包括紫外法在内的其他方法所获得的活性一致。本方法还应用于测定来自人类受试者、大鼠和小鼠的完整红细胞清除过氧化氢的速率。数据表明正常红细胞具有清除细胞外过氧化氢的显著能力。通过测量用3-氨基-1,2,4-三唑处理的红细胞中的过氧化氢酶活性以及红细胞清除过氧化氢的速率，推断出正常和无过氧化氢酶血症红细胞中过氧化氢酶清除过氧化氢的与血红蛋白含量相关的速率常数（k/g Hb）分别为42.0±6.0和8.0±3.0。
