Masuoka Noriyoshi, Zukeran Ayumi, Takemoto Kazunori, Wang Da-Hong, Ishihara Kohji
Tsudaka-Fruit Juice Laboratory, Okayama Research Park Incubation Center, 5303 Haga, Kita-ku, Okayama 701-1221, Japan.
Department of Life Science, Okayama University of Science, Okayama 700-0005, Japan.
Toxicol Rep. 2020 Feb 7;7:282-287. doi: 10.1016/j.toxrep.2020.02.001. eCollection 2020.
Normal and acatalasemic mouse erythrocytes were used to clarify the relationship between oxidative damage in HO-treated erythrocytes and catalase activity.
DESIGN & METHODS: Generation of hydrolysis-resistant erythrocytes and hemolysis were examined. The osmotic fragility test, the negative charges and the number of membrane-flickering erythrocytes among the HO-treated erythrocytes were investigated.
Small amounts of hydrolysis-resistant mouse erythrocytes were generated by treatment with 0.1 mM HO and the amount of acatalasemic erythrocytes was larger than untreated controls. Hemolysis in the acatalasemic erythrocytes was observed 30 min after the addition of the HO. A drastic increase in hydrolysis-resistant erythrocytes and a loss of membrane proteins in the acatalasemic erythrocytes were found as a result of the addition of 1 mM HO. Hemolysis in normal erythrocytes was observed at 3 mM HO.
Catalase is a potent HO-scavenger even in acatalasemic mouse erythrocytes. It is concluded that the drastic increase of hydrolysis-resistant erythrocytes is induced by a loss of membrane function and is associated with the low catalase activity in these cells.
使用正常和无过氧化氢酶小鼠红细胞来阐明经羟基脲（HO）处理的红细胞中的氧化损伤与过氧化氢酶活性之间的关系。
检测抗水解红细胞的生成及溶血情况。研究了经HO处理的红细胞的渗透脆性试验、负电荷以及膜闪烁红细胞的数量。
用0.1 mM HO处理可产生少量抗水解小鼠红细胞，且无过氧化氢酶红细胞的数量比未处理的对照组多。添加HO后30分钟，在无过氧化氢酶红细胞中观察到溶血现象。添加1 mM HO后，发现无过氧化氢酶红细胞中抗水解红细胞急剧增加且膜蛋白丢失。在3 mM HO时观察到正常红细胞溶血。
即使在无过氧化氢酶小鼠红细胞中，过氧化氢酶也是一种有效的HO清除剂。得出结论，抗水解红细胞的急剧增加是由膜功能丧失引起的，并且与这些细胞中低过氧化氢酶活性有关。
