Adams E F, Todo T, Schrell U M, Thierauf P, White M C, Fahlbusch R
Department of Neurosurgery, University of Erlangen-Nuremberg, Germany.
Int J Cancer. 1991 Sep 30;49(3):398-402. doi: 10.1002/ijc.2910490315.
We have used cell-culture techniques to investigate growth-factor production by human meningioma cells. Meningioma tissue was dispersed with collagenase and the cells grown to high density in tissue-culture flasks. The cultures were used to generate conditioned medium (MEN-CM), which was used to cultivate IMR32 cells (a human neuroblastoma line) and freshly dispersed primary meningioma cells. MEN-CM profoundly stimulated the in vitro growth of both IMR32 and meningioma cells. In addition, H3-thymidine uptake by cultured meningioma cells was increased in a dose-dependent manner by varying concentrations of MEN-CM. A neutralizing anti-body against platelet-derived growth factor (PDGF) completely abolished the stimulatory effects of MEN-CM, whereas an antibody against TGF-alpha was without effect. The mitogenic activity of MEN-CM, as assayed by promotion of H3-thymidine uptake by cultured meningioma cells, eluted from a Sephadex G-100 column in 3 peaks corresponding to molecular weights of greater than or equal to 150, 56 and 28 kDa. Our results show that proliferation of human meningiomas may be under autocrine control via secretion of PDGF-like molecules.
我们运用细胞培养技术来研究人类脑膜瘤细胞生长因子的产生情况。用胶原酶分散脑膜瘤组织，然后将细胞接种于组织培养瓶中，使其生长至高密度。这些培养物用于制备条件培养基（MEN-CM），该条件培养基被用来培养IMR32细胞（一种人类神经母细胞瘤细胞系）和新分散的原发性脑膜瘤细胞。MEN-CM能显著刺激IMR32细胞和脑膜瘤细胞的体外生长。此外，不同浓度的MEN-CM能以剂量依赖的方式增加培养的脑膜瘤细胞对³H-胸苷的摄取。一种针对血小板衍生生长因子（PDGF）的中和抗体能完全消除MEN-CM的刺激作用，而针对转化生长因子-α（TGF-α）的抗体则没有效果。通过检测培养的脑膜瘤细胞对³H-胸苷摄取的促进作用来测定，MEN-CM的促有丝分裂活性从Sephadex G-100柱上洗脱下来呈现3个峰，分别对应分子量大于或等于150 kDa、56 kDa和28 kDa。我们的结果表明，人类脑膜瘤的增殖可能通过分泌类PDGF分子受到自分泌控制。
