van Dijk Jeroen P, Cankar Katarina, Scheffer Stanley J, Beenen Henriek G, Shepherd Louise V T, Stewart Derek, Davies Howard V, Wilkockson Steve J, Leifert Carlo, Gruden Kristina, Kok Esther J
RIKILT Institute of Food Safety, Bornsesteeg 45, P.O. Box 230, 6700 AE Wageningen, The Netherlands.
J Agric Food Chem. 2009 Feb 25;57(4):1612-23. doi: 10.1021/jf802815d.
The use of profiling techniques such as transcriptomics, proteomics, and metabolomics has been proposed to improve the detection of side effects of plant breeding processes. This paper describes the construction of a food safety-oriented potato cDNA microarray (FSPM). Microarray analysis was performed on a well-defined set of tuber samples of two different potato varieties, grown under different, well-recorded environmental conditions. Data were analyzed to assess the potential of transcriptomics to detect differences in gene expression due to genetic differences or environmental conditions. The most pronounced differences were found between the varieties Sante and Lady Balfour, whereas differences due to growth conditions were less significant. Transcriptomics results were confirmed by quantitative PCR. Furthermore, the bandwidth of natural variation of gene expression was explored to facilitate biological and/or toxicological evaluation in future assessments.
有人提出使用转录组学、蛋白质组学和代谢组学等分析技术来改进对植物育种过程副作用的检测。本文描述了一种面向食品安全的马铃薯cDNA微阵列（FSPM）的构建。对在不同的、记录良好的环境条件下种植的两个不同马铃薯品种的一组明确的块茎样本进行了微阵列分析。对数据进行分析，以评估转录组学检测由于遗传差异或环境条件导致的基因表达差异的潜力。在Sante和Lady Balfour品种之间发现了最明显的差异，而由于生长条件导致的差异则不太显著。转录组学结果通过定量PCR得到证实。此外，还探索了基因表达自然变异的带宽，以便在未来评估中促进生物学和/或毒理学评估。
