Zhang D, Seno S, Akita M, Tsujii T
Division of Pathology, Shigei Medical Research Institute, Okayama, Japan.
Histochemistry. 1991;96(2):115-21. doi: 10.1007/BF00315981.
For the purpose of revealing the barrier effect of the anionic groups of glomerular capillary wall against the serum protein leakage, morphologic and histochemical observations were made on the rat kidney perfused in situ with three kinds of cationic macromolecules different in chemical characteristics followed by blood flow restoration. The polyethyleneimine perfusion resulted in the complete disapperance of ionized anionic groups of glomerular capillary and the massive protein leakage through glomeruli by blood flow restoration. Cationic ferric colloid perfusion induced moderate protein leakage, and avidin perfusion was less in neutralization effect of anionic groups and the protein leakage was of least. The protein leakage from glomeruli, however, was stopped or markedly suppressed soon after the blood flow restoration by the newly formed functioning anionic barrier probably by some particular serum protein deposition. The findings indicate that the deionization of the glomerular capillary wall will not be responsible for the persistent albuminuria.
为了揭示肾小球毛细血管壁阴离子基团对血清蛋白渗漏的屏障作用，对原位灌注三种化学特性不同的阳离子大分子后再恢复血流的大鼠肾脏进行了形态学和组织化学观察。聚乙烯亚胺灌注导致肾小球毛细血管离子化阴离子基团完全消失，恢复血流后大量蛋白质通过肾小球渗漏。阳离子铁胶体灌注引起中度蛋白质渗漏，抗生物素蛋白灌注对阴离子基团的中和作用较小，蛋白质渗漏最少。然而，恢复血流后，新形成的起作用的阴离子屏障（可能是由于某些特定血清蛋白沉积）很快使肾小球蛋白质渗漏停止或明显受到抑制。这些发现表明，肾小球毛细血管壁的去离子化并非持续性蛋白尿的原因。
