Zhang Yi, Liu Ruijin, Hu Yuling, Li Gongke
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Anal Chem. 2009 Feb 1;81(3):967-76. doi: 10.1021/ac8018262.
The use of microwave heating for preparation in organic synthesis has been accepted as an effective technique in synthesis due to the significant advantages over the conventional method. In the present work, microwave heating was applied to the preparation of atrazine (template molecule) magnetic molecularly imprinted polymer (mag-MIP) beads by suspension polymerization. The term of the polymerization was dramatically shortened by using microwave heating in polymerization, which was less than (1)/(10) that by conventional heating. The resultant polymers incorporating molecular recognition and magnetic separation can provide a highly selective material for trace analysis in complicated samples. The mag-MIP beads were demonstrated with a narrow diameter distribution (80-250 microm) and cross-linking, spherical shape, and porous morphologies and exhibited magnetic property (M(s) = 0.491 emicro/g) and thermal stability under 260 degrees C. An improvement of imprinted efficiency is obtained in comparison to the mag-MIP beads prepared by conventional heating. A method for the determination of triazines in complicated samples by the mag-MIP beads extraction coupled with high-performance liquid chromatography (HPLC) was developed. The results indicated that the mag-MIP beads can be favorably used for the extraction of the triazines in spiked soil, soybean, lettuce, and millet samples. The reused beads displayed a long-term stability after undergoing extraction of 100 times.
在有机合成中，由于微波加热相对于传统方法具有显著优势，其已被公认为一种有效的合成技术。在本研究中，通过悬浮聚合法将微波加热应用于制备阿特拉津（模板分子）磁性分子印迹聚合物（mag-MIP）微球。在聚合过程中使用微波加热极大地缩短了聚合时间，该时间不到传统加热时间的1/10。所得的兼具分子识别和磁分离功能的聚合物可为复杂样品中的痕量分析提供一种高选择性材料。mag-MIP微球具有窄的粒径分布（80 - 250微米），呈交联球形且具有多孔形态，在260℃下表现出磁性（M(s) = 0.491 emicro/g）和热稳定性。与传统加热制备的mag-MIP微球相比，印迹效率有所提高。开发了一种利用mag-MIP微球萃取结合高效液相色谱（HPLC）测定复杂样品中三嗪类的方法。结果表明，mag-MIP微球可很好地用于加标土壤、大豆、生菜和小米样品中三嗪类的萃取。重复使用的微球在经过100次萃取后仍显示出长期稳定性。
