Rankin W Velando, Henry C J, Turnquist S E, Turk J R, Beissenherz M E, Tyler J W, Rucker E B, Knapp D W, Rodriguez C O, Green J A
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri-Columbia, Columbia, MO, USA.
Vet Comp Oncol. 2008 Sep;6(3):141-50. doi: 10.1111/j.1476-5829.2007.00150.x.
Survivin, an inhibitor of apoptosis, is overexpressed in human invasive transitional cell carcinoma (TCC) of the urinary bladder. Survivin expression in canine TCC has not been defined. This study was designed to compare survivin expression between canine TCC and normal urinary bladder tissue. Reverse-transcriptase polymerase chain reaction (PCR) and immunohistochemistry (IHC) were performed on fresh-frozen and formalin-fixed tissues, respectively. All TCC tissues (n = 6) and 11/22 normal tissues assessed by PCR were positive for survivin. This difference was not significant (P = 0.06). With regard to IHC, 28/41 TCC samples were positive for nuclear survivin, whereas 0/46 normal tissues had nuclear immunoreactivity (P < 0.001). Cytoplasmic immunoreactivity did not significantly differ between TCC (7/41) and normal tissues (17/46) (P = 0.07). We conclude that nuclear survivin is present in canine TCC, but not in normal bladder urothelium. Future studies will evaluate the role of nuclear survivin in TCC development and as a potential therapeutic target.
生存素是一种凋亡抑制因子，在人类膀胱浸润性移行细胞癌（TCC）中过度表达。犬TCC中生存素的表达尚未明确。本研究旨在比较犬TCC与正常膀胱组织中生存素的表达情况。分别对新鲜冷冻组织和福尔马林固定组织进行逆转录聚合酶链反应（PCR）和免疫组织化学（IHC）检测。通过PCR评估的所有TCC组织（n = 6）和11/22正常组织生存素呈阳性。这种差异不显著（P = 0.06）。关于IHC，28/41 TCC样本核生存素呈阳性，而0/46正常组织有核免疫反应性（P < 0.001）。TCC（7/41）和正常组织（17/46）之间的细胞质免疫反应性无显著差异（P = 0.07）。我们得出结论，核生存素存在于犬TCC中，但不存在于正常膀胱尿路上皮中。未来的研究将评估核生存素在TCC发展中的作用以及作为潜在治疗靶点的作用。
