Márquez-Valadez Berenice, Lugo-Huitrón Rafael, Valdivia-Cerda Verónica, Miranda-Ramírez Luis Rubén, Pérez-De La Cruz Verónica, González-Cuahutencos Octavio, Rivero-Cruz Isabel, Mata Rachel, Santamaría Abel, Pedraza-Chaverrí José
Laboratorio de Aminoácidos Excitadores, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Insurgentes Sur México DF, Mexico.
Nutr Neurosci. 2009 Feb;12(1):35-42. doi: 10.1179/147683009X388850.
The antiperoxidative properties of alpha-mangostin, a xanthone isolated from mangosteen fruit, were tested for the first time in nerve tissue exposed to different toxic insults. Two reliable biological preparations (rat brain homogenates and synaptosomal P2 fractions) were exposed to the toxic actions of a free radical generator (ferrous sulfate), an excitotoxic agent (quinolinate), and a mitochondrial toxin (3-nitropropionate). alpha-Mangostin decreased the lipoperoxidative action of FeSO(4) in both preparations in a concentration-dependent manner, and completely abolished the peroxidative effects of quinolinate, 3-nitropropionate and FeSO(4) + quinolinate at all concentrations tested. Interestingly, when tested alone in brain homogenates, alpha-mangostin significantly decreased the lipoperoxidation even below basal levels. alpha-Mangostin also prevented the decreased reductant capacity of mitochondria in synaptosomal fractions. Our results suggest that alpha-mangostin exerts a robust antiperoxidative effect in brain tissue preparations probably through its properties as a free radical scavenger. In light of these findings, this antioxidant should be tested in other neurotoxic models involving oxidative stress.
α-倒捻子素是从山竹果中分离出的一种氧杂蒽酮，其抗过氧化特性首次在遭受不同毒性损伤的神经组织中进行了测试。两种可靠的生物制剂（大鼠脑匀浆和突触体P2组分）分别暴露于自由基生成剂（硫酸亚铁）、兴奋性毒性剂（喹啉酸）和线粒体毒素（3-硝基丙酸）的毒性作用之下。α-倒捻子素在两种制剂中均以浓度依赖的方式降低了硫酸亚铁的脂质过氧化作用，并在所有测试浓度下完全消除了喹啉酸、3-硝基丙酸以及硫酸亚铁+喹啉酸的过氧化作用。有趣的是，当单独在脑匀浆中进行测试时，α-倒捻子素甚至使脂质过氧化作用显著降低至基础水平以下。α-倒捻子素还防止了突触体组分中线粒体还原能力的下降。我们的结果表明，α-倒捻子素可能通过其作为自由基清除剂的特性，在脑组织制剂中发挥强大的抗过氧化作用。鉴于这些发现，这种抗氧化剂应在其他涉及氧化应激的神经毒性模型中进行测试。
