一种均一糖蛋白酶的半合成：核糖核酸酶C：第2部分
Semisynthesis of a homogeneous glycoprotein enzyme: ribonuclease C: part 2.
作者信息
Piontek Christian, Varón Silva Daniel, Heinlein Christian, Pöhner Claudia, Mezzato Stefano, Ring Petra, Martin Andreas, Schmid Franz Xaver, Unverzagt Carlo
机构信息
Bioorganische Chemie, Gebäude NW1, Universität Bayreuth, 95440 Bayreuth, Germany.
出版信息
Angew Chem Int Ed Engl. 2009;48(11):1941-5. doi: 10.1002/anie.200804735.
PMID:19180621
Abstract
Active RNase glycoprotein from three pieces: The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N-glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme.
摘要
由三部分组成的活性核糖核酸酶糖蛋白
糖蛋白酶核糖核酸酶C含有复杂的糖类N-聚糖，通过连续的天然化学连接法合成。优化的连接和分离方案使这种含124个氨基酸的酶能够高效组装和重折叠。
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