Laich Federico S, Alcoba-Flórez Julia, Pérez-Roth Eduardo, Bahaya Yasmin, Delgado José Luis, Méndez-Alvarez Sebastián
Unidad de Investigación, Asociada al Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.
Med Mycol. 2008 Sep;46(6):615-9. doi: 10.1080/13693780801946593.
A case of fungal keratitis is presented in which corneal scrapings were obtained for microbiological studies, including morphological identification and molecular characterization of the etiologic agent. Comparative sequence analyses of the Internal Transcribed Spacer domain of 5.8S and 26S regions of nuclear rDNA showed 100% identity with different species of Alternaria and PCR-RFLP analysis of Intergenic Spacer regions revealed intraspecific variation. The combination of morphological and molecular characters resulted in the unambiguous identification of the causal agent as Alternaria alternata. Treatment with antifungals contributed to the improvement in the patient's lesions.
本文报告了一例真菌性角膜炎病例，该病例采集了角膜刮片进行微生物学研究，包括病原体的形态学鉴定和分子特征分析。对核糖体DNA的5.8S和26S区域的内转录间隔区进行的比较序列分析显示，与链格孢属的不同种具有100%的同一性，对基因间隔区的PCR-RFLP分析揭示了种内变异。形态学和分子特征的结合明确鉴定出病原体为链格孢。抗真菌药物治疗有助于患者病变的改善。
