[巴戟天抗抑郁提取物含量测定及微量元素分析]
[Determination content of the antidepressant extraction and analysis the trace elements from Morinda officinalis].
作者信息
Li Jun, Zhang Hua-Lin, Wang Zhe, Liang Yu-Ming, Jiang Lin, Ma Wei, Yang De-Po
机构信息
Institute of Natural Medicine and TCM, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510080, China.
出版信息
Zhong Yao Cai. 2008 Sep;31(9):1337-40.
OBJECTIVE
To study on the antidepressive extraction and determinated the content of the oligosaccharides I , the proteins and the trace elements from Morinda officinalis.
METHODS
We analyzed the content of the total oligosaccharides, the proteins and the trace elements by Phenol-Sulferic acid, Coomassie brilliant blue and inductive coupling plasm atomic emission spectrometer (ICP-AES) methods, respectively.
RESULTS
The percent of the oligosaccharides and the proteins were 89.4% and 0.19% in the extraction from M. officinalis, respectively. The total actively oligosaccharides I were subjected to the D-941 ion column chromatography to give the extraction in which hardly and protein were determined. 12 kinds of trace element including Zn, Fe, Ca ect. in the extraction (the oligosaccharides I) were determined by ICP-AES without the noxious trace elements including As, Cd, Pb ect.
CONCLUSION
The antidepressive activity extraction(the oligosaccharides I ) was a highly pure and vegetal oligosaccharides.
目的
研究巴戟天的抗抑郁提取物，并测定其低聚糖I、蛋白质及微量元素的含量。
方法
分别采用苯酚-硫酸法、考马斯亮蓝法和电感耦合等离子体原子发射光谱仪（ICP-AES）法分析总低聚糖、蛋白质和微量元素的含量。
结果
巴戟天提取物中低聚糖和蛋白质的含量分别为89.4%和0.19%。将总活性低聚糖I进行D-941离子柱色谱分离，得到几乎不含蛋白质的提取物。采用ICP-AES法测定提取物（低聚糖I）中包括锌、铁、钙等在内的12种微量元素，未检出包括砷、镉、铅等在内的有害微量元素。
结论
抗抑郁活性提取物（低聚糖I）是一种高纯度的植物低聚糖。
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