[硫酸铵跨膜梯度法制备马钱子碱脂质体的影响因素]
[Influencing factors in preparation of brucine liposomes by ammonium sulfate transmembrane gradients].
作者信息
Wang Wei, Chen Jun, Cai Bao-Chang, Fang Yun
机构信息
Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
出版信息
Zhong Yao Cai. 2008 Sep;31(9):1410-2.
OBJECTIVE
To study the influencing factors in preparation of brucine liposomes by ammonium sulfate transmembrane gradients.
METHODS
The brucine liposomes were separated by Sephadex G-50, and the influence of various factors on the entrapment efficiencies were investigated.
RESULTS
The entrapment efficiency was enhanced by increased ammonium sulfate concentration, ethanol volume and PC concentration.
CONCLUSION
Burcine liposomes prepared by ammonium sulfate transmembrance gradients can get a high entrapment efficiency, the main influencing factors were ammonium sulfate concentration, ethanol volume and PC concentration.
目的
研究采用硫酸铵跨膜梯度法制备马钱子碱脂质体的影响因素。
方法
采用葡聚糖凝胶G - 50分离马钱子碱脂质体，考察各种因素对包封率的影响。
结果
随着硫酸铵浓度、乙醇体积和磷脂酰胆碱（PC）浓度的增加，包封率提高。
结论
采用硫酸铵跨膜梯度法制备的马钱子碱脂质体可获得较高的包封率，主要影响因素为硫酸铵浓度、乙醇体积和PC浓度。
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