de la Escosura-Muñiz Alfredo, Maltez-da Costa Marisa, Merkoçi Arben
Nanobioelectronics & Biosensors Group, Institut Català de Nanotecnologia, Barcelona, Spain.
Biosens Bioelectron. 2009 Apr 15;24(8):2475-82. doi: 10.1016/j.bios.2008.12.028. Epub 2008 Dec 25.
An electrocatalytical method induced by gold nanoparticles in order to improve the sensitivity of the magnetoimmunosensing technology is reported. Microparamagnetic beads as primary antibodies immobilization platforms and gold nanoparticles modified with secondary antibodies as high sensitive electrocatalytical labels are used. A built-in magnet carbon electrode allows the collection/immobilization on its surface of the microparamagnetic beads with the immunological sandwich and gold nanoparticle catalysts attached onto. The developed magnetoimmunosensing technology allows the antigen detection with an enhanced sensitivity due to the catalytic effect of gold nanoparticles on the electroreduction of silver ions. The main parameters that affect the different steps of the developed assay are optimized so as to reach a high sensitive electrochemical detection of the protein. The low levels of gold nanoparticles detected with this method allow the obtaining of a novel immunosensor with low protein detection limits (up to 23 fg/mL), with special interest for further applications in clinical analysis, food quality and safety as well as other industrial applications.
报道了一种由金纳米颗粒诱导的电催化方法，以提高磁免疫传感技术的灵敏度。使用微顺磁珠作为一抗固定平台，并用二抗修饰的金纳米颗粒作为高灵敏度电催化标记物。内置磁碳电极允许通过免疫夹心将微顺磁珠及其上附着的金纳米颗粒催化剂收集/固定在其表面。由于金纳米颗粒对银离子电还原的催化作用，所开发的磁免疫传感技术能够以更高的灵敏度检测抗原。对影响所开发检测方法不同步骤的主要参数进行了优化，以实现对蛋白质的高灵敏度电化学检测。用该方法检测到的低水平金纳米颗粒使得能够获得一种新型免疫传感器，其蛋白质检测限低（高达23 fg/mL），在临床分析、食品质量和安全以及其他工业应用等进一步应用中具有特殊意义。
