Nelson William, Soderlund Carol
Arizona Genomics Computational Laboratory, BIO5 Institute, University of Arizona, Tucson, AZ, USA.
Nucleic Acids Res. 2009 Apr;37(5):e36. doi: 10.1093/nar/gkp034. Epub 2009 Jan 30.
Recent advances in both clone fingerprinting and draft sequencing technology have made it increasingly common for species to have a bacterial artificial clone (BAC) fingerprint map, BAC end sequences (BESs) and draft genomic sequence. The FPC (fingerprinted contigs) software package contains three modules that maximize the value of these resources. The BSS (blast some sequence) module provides a way to easily view the results of aligning draft sequence to the BESs, and integrates the results with the following two modules. The MTP (minimal tiling path) module uses sequence and fingerprints to determine a minimal tiling path of clones. The DSI (draft sequence integration) module aligns draft sequences to FPC contigs, displays them alongside the contigs and identifies potential discrepancies; the alignment can be based on either individual BES alignments to the draft, or on the locations of BESs that have been assembled into the draft. FPC also supports high-throughput fingerprint map generation as its time-intensive functions have been parallelized for Unix-based desktops or servers with multiple CPUs. Simulation results are provided for the MTP, DSI and parallelization. These features are in the FPC V9.3 software package, which is freely available.
克隆指纹识别技术和草图测序技术的最新进展使得物种拥有细菌人工克隆(BAC)指纹图谱、BAC末端序列(BESs)和基因组草图序列变得越来越普遍。FPC(指纹连续片段)软件包包含三个模块,可将这些资源的价值最大化。BSS(对某些序列进行比对)模块提供了一种轻松查看草图序列与BESs比对结果的方法,并将结果与以下两个模块集成。MTP(最小拼接路径)模块利用序列和指纹来确定克隆的最小拼接路径。DSI(草图序列整合)模块将草图序列与FPC连续片段进行比对,将它们与连续片段一起显示并识别潜在差异;比对可以基于单个BES与草图的比对,也可以基于已组装到草图中的BESs的位置。FPC还支持高通量指纹图谱生成,因为其耗时的功能已针对基于Unix的桌面或具有多个CPU的服务器进行了并行化处理。提供了MTP、DSI和并行化的模拟结果。这些功能在FPC V9.3软件包中,该软件包可免费获取。