Suppr超能文献

将序列与FPC指纹图谱整合。

Integrating sequence with FPC fingerprint maps.

作者信息

Nelson William, Soderlund Carol

机构信息

Arizona Genomics Computational Laboratory, BIO5 Institute, University of Arizona, Tucson, AZ, USA.

出版信息

Nucleic Acids Res. 2009 Apr;37(5):e36. doi: 10.1093/nar/gkp034. Epub 2009 Jan 30.

Abstract

Recent advances in both clone fingerprinting and draft sequencing technology have made it increasingly common for species to have a bacterial artificial clone (BAC) fingerprint map, BAC end sequences (BESs) and draft genomic sequence. The FPC (fingerprinted contigs) software package contains three modules that maximize the value of these resources. The BSS (blast some sequence) module provides a way to easily view the results of aligning draft sequence to the BESs, and integrates the results with the following two modules. The MTP (minimal tiling path) module uses sequence and fingerprints to determine a minimal tiling path of clones. The DSI (draft sequence integration) module aligns draft sequences to FPC contigs, displays them alongside the contigs and identifies potential discrepancies; the alignment can be based on either individual BES alignments to the draft, or on the locations of BESs that have been assembled into the draft. FPC also supports high-throughput fingerprint map generation as its time-intensive functions have been parallelized for Unix-based desktops or servers with multiple CPUs. Simulation results are provided for the MTP, DSI and parallelization. These features are in the FPC V9.3 software package, which is freely available.

摘要

克隆指纹识别技术和草图测序技术的最新进展使得物种拥有细菌人工克隆(BAC)指纹图谱、BAC末端序列(BESs)和基因组草图序列变得越来越普遍。FPC(指纹连续片段)软件包包含三个模块,可将这些资源的价值最大化。BSS(对某些序列进行比对)模块提供了一种轻松查看草图序列与BESs比对结果的方法,并将结果与以下两个模块集成。MTP(最小拼接路径)模块利用序列和指纹来确定克隆的最小拼接路径。DSI(草图序列整合)模块将草图序列与FPC连续片段进行比对,将它们与连续片段一起显示并识别潜在差异;比对可以基于单个BES与草图的比对,也可以基于已组装到草图中的BESs的位置。FPC还支持高通量指纹图谱生成,因为其耗时的功能已针对基于Unix的桌面或具有多个CPU的服务器进行了并行化处理。提供了MTP、DSI和并行化的模拟结果。这些功能在FPC V9.3软件包中,该软件包可免费获取。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a0b7/2655663/b829681fd416/gkp034f1.jpg

相似文献

1
Integrating sequence with FPC fingerprint maps.
Nucleic Acids Res. 2009 Apr;37(5):e36. doi: 10.1093/nar/gkp034. Epub 2009 Jan 30.
2
Locating sequence on FPC maps and selecting a minimal tiling path.
Genome Res. 2003 Sep;13(9):2152-63. doi: 10.1101/gr.1068603. Epub 2003 Aug 12.
3
Assembly of fingerprint contigs: parallelized FPC.
Bioinformatics. 2002 Mar;18(3):484-5. doi: 10.1093/bioinformatics/18.3.484.
4
FPC: a system for building contigs from restriction fingerprinted clones.
Comput Appl Biosci. 1997 Oct;13(5):523-35. doi: 10.1093/bioinformatics/13.5.523.
5
Contigs built with fingerprints, markers, and FPC V4.7.
Genome Res. 2000 Nov;10(11):1772-87. doi: 10.1101/gr.gr-1375r.
8
Dynamic building of a BAC clone tiling path for the Rat Genome Sequencing Project.
Genome Res. 2004 Apr;14(4):679-84. doi: 10.1101/gr.2171704.
9
A BAC-based physical map of the channel catfish genome.
Genomics. 2007 Sep;90(3):380-8. doi: 10.1016/j.ygeno.2007.05.008. Epub 2007 Jun 20.
10
A graph-theoretical approach to the selection of the minimum tiling path from a physical map.
IEEE/ACM Trans Comput Biol Bioinform. 2013 Mar-Apr;10(2):352-60. doi: 10.1109/TCBB.2013.26.

引用本文的文献

1
The improved assembly of 7DL chromosome provides insight into the structure and evolution of bread wheat.
Plant Biotechnol J. 2020 Mar;18(3):732-742. doi: 10.1111/pbi.13240. Epub 2019 Sep 18.
2
Young inversion with multiple linked QTLs under selection in a hybrid zone.
Nat Ecol Evol. 2017 Apr 3;1(5):119. doi: 10.1038/s41559-017-0119.
5
Cassava genome from a wild ancestor to cultivated varieties.
Nat Commun. 2014 Oct 10;5:5110. doi: 10.1038/ncomms6110.
6
A physical map of the short arm of wheat chromosome 1A.
PLoS One. 2013 Nov 21;8(11):e80272. doi: 10.1371/journal.pone.0080272. eCollection 2013.
7
Physical mapping integrated with syntenic analysis to characterize the gene space of the long arm of wheat chromosome 1A.
PLoS One. 2013 Apr 16;8(4):e59542. doi: 10.1371/journal.pone.0059542. Print 2013.

本文引用的文献

3
Toward sequencing cotton (Gossypium) genomes.
Plant Physiol. 2007 Dec;145(4):1303-10. doi: 10.1104/pp.107.107672.
4
Physical and genetic structure of the maize genome reflects its complex evolutionary history.
PLoS Genet. 2007 Jul;3(7):e123. doi: 10.1371/journal.pgen.0030123.
5
GenoProfiler: batch processing of high-throughput capillary fingerprinting data.
Bioinformatics. 2007 Jan 15;23(2):240-2. doi: 10.1093/bioinformatics/btl494. Epub 2006 Oct 2.
6
Efficacy of clone fingerprinting methodologies.
Genomics. 2007 Jan;89(1):160-5. doi: 10.1016/j.ygeno.2006.08.008. Epub 2006 Oct 2.
7
The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science. 2006 Sep 15;313(5793):1596-604. doi: 10.1126/science.1128691.
8
SyMAP: A system for discovering and viewing syntenic regions of FPC maps.
Genome Res. 2006 Sep;16(9):1159-68. doi: 10.1101/gr.5396706.
9
Physical map-assisted whole-genome shotgun sequence assemblies.
Genome Res. 2006 Jun;16(6):768-75. doi: 10.1101/gr.5090606.
10
Toward a physical map of the genome of the nematode Caenorhabditis elegans.
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7821-5. doi: 10.1073/pnas.83.20.7821.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验