State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, China.
Appl Biochem Biotechnol. 2009 Dec;159(3):696-707. doi: 10.1007/s12010-009-8525-z. Epub 2009 Jan 28.
This study examined the inhibition performance by the major lignocellulose degradation products, including organic acids, furan derivatives, lignin derivatives, and ethanol, on a broadly used commercial cellulase enzyme Spezyme CP (Genencor International, Rochester, NY, USA) to cellulose hydrolysis at both the well-mixing state (shaking flask) and the static state (test tube). The cellulase activity in the cellulase complex of Spezyme CP was assumed to be one single "cellulase", and the apparent kinetic parameters of this cellulase enzyme were measured as an approximate index of the inhibitory effect to the industrial cellulase enzyme. The inhibition performance of these degradation products was compared and analyzed using the determined apparent kinetic parameters. All the degradation products strongly inhibit the cellulose hydrolysis by cellulase enzyme, and the inhibitions on cellulase were all competitive type. The order of the inhibition strength by the lignocellulose degradation products to cellulase is lignin derivatives > furan derivatives > organic acids > ethanol. This study gave a quantitative view to the enzymatic hydrolysis of lignocellulose under the inhibition performance of the lignocellulose degradation products and will help to understand the lignocellulose recalcitrance to enzyme hydrolysis.
本研究考察了主要木质纤维素降解产物（包括有机酸、呋喃衍生物、木质素衍生物和乙醇）对广泛使用的商业纤维素酶 Spezyme CP（美国罗彻斯特 Genencor International）在均相（摇瓶）和静态（试管）条件下对纤维素水解的抑制性能。Spezyme CP 纤维素酶复合物中的纤维素酶活性被假定为单一的“纤维素酶”，并测量该纤维素酶的表观动力学参数作为工业纤维素酶的抑制效果的近似指标。使用确定的表观动力学参数比较和分析了这些降解产物的抑制性能。所有降解产物均强烈抑制纤维素酶对纤维素的水解，对纤维素酶的抑制均为竞争性抑制。木质纤维素降解产物对纤维素酶的抑制强度顺序为木质素衍生物>呋喃衍生物>有机酸>乙醇。本研究从定量的角度考察了木质纤维素降解产物抑制性能下木质纤维素的酶解，并有助于理解木质纤维素对酶解的顽固性。
