Suryanarayanan Trichur Subramanian, Rajamani Thavamani, Aro Nina, Borisova Anna, Marjamaa Kaisa, Govindarajulu Meenavalli Babu
Vivekananda Institute of Tropical Mycology, Ramakrishna Mission Vidyapith, Chennai, 600004 India.
VTT Technical Research Centre of Finland Ltd, P.O. Box 1000, 020244 VTT Espoo, Finland.
3 Biotech. 2024 Oct;14(10):243. doi: 10.1007/s13205-024-04087-3. Epub 2024 Sep 23.
Hydrothermal pretreatments are commonly employed prior to the biotechnological conversion of lignocellulosic biomass (LCB) into value-added products, such as fuels and chemicals. However, the by-products of this pretreatment, including furaldehydes, lignin-derived phenolics, and carboxylic acids, can inhibit the enzymes and microbes used in the biotechnological process. In this study, LCB degrading enzymes of endophytic and litter fungi were screened for their tolerance to potential pretreatment-derived inhibitors. Several fungi produced endo- and exoglucanases that remained functional in the presence of lignocellulose-derived phenolics. Some were also active in the presence of tannic acid. Additionally, thermostable endoglucanase activity was observed in some fungi. The ability of some of these fungi to utilize furaldehyde inhibitors as a sole carbon source was also noted. The culture supernatants of the fungal strains were tested in hydrolysis experiments using microcrystalline cellulose as a substrate, in the presence of lignocellulose phenolics and tannic acid. With some strains, higher sugar yields were obtained in the hydrolysis of cellulose when phenolics were added. Our results highlight the need for more intensive exploration of endophytic and plant litter fungi for novel inhibitor-resistant cellulases for biofuel production.
The online version contains supplementary material available at 10.1007/s13205-024-04087-3.
在将木质纤维素生物质（LCB）生物技术转化为增值产品（如燃料和化学品）之前，通常会采用水热预处理。然而，这种预处理的副产物，包括糠醛、木质素衍生的酚类和羧酸，会抑制生物技术过程中使用的酶和微生物。在本研究中，筛选了内生真菌和凋落物真菌的LCB降解酶对潜在预处理衍生抑制剂的耐受性。几种真菌产生的内切和外切葡聚糖酶在木质纤维素衍生的酚类存在下仍保持活性。有些在单宁酸存在下也具有活性。此外，在一些真菌中观察到了耐热内切葡聚糖酶活性。还注意到其中一些真菌能够将糠醛抑制剂作为唯一碳源利用。在木质纤维素酚类和单宁酸存在的情况下，使用微晶纤维素作为底物，对真菌菌株的培养上清液进行水解实验。对于一些菌株，添加酚类物质时，纤维素水解可获得更高的糖产量。我们的结果强调，需要更深入地探索内生真菌和植物凋落物真菌，以寻找用于生物燃料生产的新型抗抑制剂纤维素酶。
在线版本包含可在10.1007/s13205-024-04087-3获取的补充材料。
