Department of Hygiene and Preventive Medicine, Faculty of Veterinary Medicine, Kafer Elshikh University, Kafrelsheikh 33516, Egypt.
Unit of Biotechnology, Division of Biological and Life Sciences, Graduate School of Integrated Sciences for Life, Hiroshima University, 1-3-1 Kagamiyama, Higashi-Hiroshima 739-8530, Japan.
Int J Environ Res Public Health. 2021 Nov 15;18(22):11988. doi: 10.3390/ijerph182211988.
In this study, marine sediment (MS) was successfully used as a source of methanogenic bacteria for the anaerobic digestion (AD) of chicken manure (CM). Using MS showed high production in liquid and semi-solid conditions. Even in solid conditions, 169.3 mL/g volatile solids of chicken manure (VS-CM) was produced, despite the accumulation of ammonia (4.2 g NH-N/kg CM). To the best of our knowledge, this is the highest methane production from CM alone, without pretreatment, in solid conditions (20%). Comparing MS to Ozouh sludge (excess activated sewage sludge) (OS), using OS under semi-solid conditions resulted in higher methane production, while using MS resulted in more ammonia tolerance (301 mL/gVS-CM at 8.58 g NH-N/kg). Production optimization was carried out via a response surface methodology (RDM) model involving four independent variables (inoculum ratio, total solid content, NaCl concentration, and incubation time). Optimized methane production (324.36 mL/gVS-CM) was at a CM:MS ratio of 1:2.5 with no NaCl supplementation, 10% total solid content, and an incubation time of 45 days.
在本研究中，海洋沉积物（MS）成功地被用作产甲烷菌的来源，用于鸡粪（CM）的厌氧消化（AD）。使用 MS 显示出在液体和半固体条件下的高产量。即使在固体条件下，也产生了 169.3 毫升/克鸡粪挥发性固体（VS-CM），尽管氨（4.2 克 NH-N/千克 CM）积累。据我们所知，这是在没有预处理的情况下，在固体条件下（20%）单独从鸡粪中获得的最高甲烷产量。与 Ozouh 污泥（过量活性污水污泥）（OS）相比，在半固体条件下使用 OS 导致更高的甲烷产量，而使用 MS 导致更高的氨容忍度（301 毫升/克 VS-CM，在 8.58 克 NH-N/千克）。通过涉及四个独立变量（接种物比、总固体含量、NaCl 浓度和孵育时间）的响应面方法（RDM）模型进行了生产优化。优化后的甲烷产量（324.36 毫升/克 VS-CM）在没有 NaCl 补充、总固体含量为 10%和孵育时间为 45 天的条件下，CM:MS 比为 1:2.5。
