Stabel J R, Reinhardt T A, Nonnecke B J
National Animal Disease Center, USDA, Ames, IA 50010.
J Dairy Sci. 1991 Aug;74(8):2501-6. doi: 10.3168/jds.S0022-0302(91)78427-0.
A series of experiments was conducted to evaluate the effects of inorganic and organic forms of Se with or without reducing agents on in vitro IgM production by bovine lymphocytes. Peripheral mononuclear cells were isolated from nonlactating Jersey cows fed a diet with adequate Se. Cells were stimulated with pokeweed mitogen and, in addition, were cultured with various Se compounds at a concentration of 100 ng Se/ml. Mercaptoethanol (50 microM) and glutathione (1 mM) were included in cultures of cells stimulated by pokeweed mitogen with and without inorganic Se. Sodium selenite was less effective than selenomethionine and selenocystine in augmenting pokeweed mitogen-induced Ig synthesis. The addition of mercaptoethanol to pokeweed mitogen-stimulated control cultures enhanced in vitro IgM production, whereas the addition of glutathione had a negligible effect, but addition of either in combination with sodium selenite dramatically depressed IgM production. These results suggest that Se in inorganic or organic forms enhances B-cell function in vitro.
进行了一系列实验，以评估有无还原剂时无机和有机形式的硒对牛淋巴细胞体外产生IgM的影响。从饲喂含充足硒日粮的非泌乳泽西奶牛中分离外周血单核细胞。用商陆有丝分裂原刺激细胞，此外，细胞还与浓度为100 ng硒/毫升的各种硒化合物一起培养。在有和没有无机硒的情况下，商陆有丝分裂原刺激的细胞培养物中都加入了巯基乙醇（50 microM）和谷胱甘肽（1 mM）。亚硒酸钠在增强商陆有丝分裂原诱导的Ig合成方面不如硒代蛋氨酸和硒代胱氨酸有效。向商陆有丝分裂原刺激的对照培养物中添加巯基乙醇可增强体外IgM的产生，而添加谷胱甘肽的作用可忽略不计，但与亚硒酸钠联合添加则会显著降低IgM的产生。这些结果表明，无机或有机形式的硒在体外可增强B细胞功能。
