Pryjma J, Muñoz J, Galbraith R M, Fudenberg H H, Virella G
J Immunol. 1980 Feb;124(2):656-61.
The synthesis and secretion of immunoglobulins by human peripheral blood mononuclear cells in cultures stimulated with pokeweed mitogen or Staphylococcus aureus Cowan I were evaluated by enumeration of cells containing cytoplasmic immunoglobulins and cells actively secreting immunoglobulins, and by quantitation of immunoglobulins released into culture supernatants. The two mitogens caused comparable stimulation of immunoglobulin production; however, in contrast to pokeweed mitogen, S. aureus was active in cultures depleted of T lymphocytes, and its stimulatory effects were resistant to the influence of suppressor T cells generated by co-stimulation with concanavalin A or by preincubation without mitogenic stimulus. These results indicate distinct pathways of induction and suppression of immunoglobulin synthesis for these two polyclonal B cell activators, and suggest that stimulation by S. aureus is less thymus dependent than that induced by pokeweed mitogen.
通过对含有细胞质免疫球蛋白的细胞和积极分泌免疫球蛋白的细胞进行计数，以及对释放到培养上清液中的免疫球蛋白进行定量，评估了用商陆有丝分裂原或金黄色葡萄球菌Cowan I刺激的培养物中人类外周血单核细胞免疫球蛋白的合成和分泌。这两种有丝分裂原对免疫球蛋白的产生具有相当的刺激作用；然而，与商陆有丝分裂原不同，金黄色葡萄球菌在T淋巴细胞耗竭的培养物中具有活性，并且其刺激作用不受与伴刀豆球蛋白A共同刺激或无有丝分裂原刺激预孵育产生的抑制性T细胞的影响。这些结果表明这两种多克隆B细胞激活剂在免疫球蛋白合成的诱导和抑制途径上存在差异，并表明金黄色葡萄球菌的刺激比商陆有丝分裂原诱导的刺激对胸腺的依赖性更小。
