Lu Gang, Dolgner Stephen J, Hall Traci M Tanaka
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, United States of America.
Curr Opin Struct Biol. 2009 Feb;19(1):110-5. doi: 10.1016/j.sbi.2008.12.009. Epub 2009 Jan 29.
PUF proteins are RNA-binding proteins named for founding members PUMILIO and fem-3 binding factor (FBF). Together these proteins represent the range of known RNA recognition properties. PUMILIO is a prototypical PUF protein whose RNA sequence specificity is simple, elegant, and predictable. FBF displays differences in RNA recognition that represent divergence from the prototype. Here we review recent studies that examine the engineering of sequence specificity of PUF proteins and its applications as well as studies that increase our understanding of the natural diversity of RNA recognition by this family of proteins.
PUF蛋白是一类RNA结合蛋白,得名于其最初的成员——PUMILIO和fem-3结合因子(FBF)。这些蛋白共同代表了已知的RNA识别特性范围。PUMILIO是一种典型的PUF蛋白,其RNA序列特异性简单、优雅且可预测。FBF在RNA识别方面表现出差异,代表了与原型的分歧。在这里,我们回顾了最近关于研究PUF蛋白序列特异性工程及其应用的研究,以及那些增进我们对该蛋白家族RNA识别自然多样性理解的研究。