Western Australian Institute for Medical Research and Centre for Medical Research, The University of Western Australia, Perth, Australia.
Nat Chem Biol. 2011 May 15;7(7):425-7. doi: 10.1038/nchembio.577.
The design of proteins that can bind any RNA sequence of interest has many potential biological and medical applications. Here we have expanded the recognition of Pumilio and FBF homology protein (PUF) repeats beyond adenine, guanine and uracil and evolved them to specifically bind cytosine. These repeat sequences can be used to create PUF domains capable of selectively binding RNA targets of diverse sequence and structure.
设计能够结合任何感兴趣的 RNA 序列的蛋白质具有许多潜在的生物学和医学应用。在这里，我们扩展了 Pumilio 和 FBF 同源蛋白 (PUF) 重复序列对腺嘌呤、鸟嘌呤和尿嘧啶的识别，并将其进化为专门结合胞嘧啶。这些重复序列可用于创建 PUF 结构域，使其能够选择性地结合具有不同序列和结构的 RNA 靶标。
