The production of hepatic cirrhosis in rats.

This study investigated the conditions necessary to produce a predictable yield of cirrhosis in rats. A previously published method, using carbon tetrachloride, was used as a basis for the study. Histopathology was used in a semi-quantitative manner to examine which observations could be used for the prediction of progression and final yield of cirrhosis. A comparison of different vehicles for oral carbon tetrachloride administration showed no effect of vehicle on the final yield of cirrhosis. Also, there were no observations during the study that should be used for prediction. Variability in the severity of hepatic impairment is an inherent feature of this model, and supporting histopathology is essential. We recommend a simple protocol for the initiation of cirrhosis in rats using carbon tetrachloride.