Suchomel Miranda, Koller Walter, Kundi Michael, Rotter Manfred L
Department of Hygiene and Medical Microbiology, Medical University Vienna, Kinderspitalgasse 15, Vienna, Austria.
Am J Infect Control. 2009 May;37(4):289-93. doi: 10.1016/j.ajic.2008.09.026. Epub 2009 Feb 1.
The recommended duration for surgical hand treatment has been changed from 10 over 5 to 3 minutes and even shorter.
Our objective was to study the impact of the length of surgical hand antisepsis with n-propanol 60% (vol/vol) or isopropanol 70% (vol/vol) applied for 1, 3, or 5 minutes on the reduction of resident hand flora in the setting of the microbiologic laboratory for experimental and applied testing of disinfectants and antiseptics at the Medical University Vienna, Austria, using a Latin Square design.
Our methods were according to the Austrian Guidelines for Testing Products for Surgical Hand Antisepsis. The release of bacterial hand flora of 21 subjects is assessed before and immediately after disinfection from one hand and 3 hours later from the other, meanwhile gloved, hand. Mean reduction factors (RF) are calculated.
The immediate mean log(10) RFs with n-propanol or isopropanol were 1.05, 2.03, and 2.30 and 0.74, 1.48, and 2.12, respectively, when applied for 1, 3, or 5 minutes, respectively. After 3 hours, the respective mean log(10) RFs were 0.45, 1.01, and 1.60 and 0.19, 0.79, and 1.03. Thus, with increasing length of application, a highly significant trend (P < .001) toward higher log(10) reductions was demonstrated. At both sampling times, n-propanol was more effective than isopropanol at the corresponding treatments. Furthermore, a highly significant (P < .001) association was found between the individual volunteers and the effect of the antiseptics on their hands.
The efficacy of surgical antisepsis is significantly associated with the length of application.
外科手部消毒的推荐时长已从10分钟超过5分钟变为3分钟甚至更短。
我们的目的是采用拉丁方设计，研究在奥地利维也纳医科大学微生物实验室环境下，使用60%（体积/体积）正丙醇或70%（体积/体积）异丙醇进行1、3或5分钟的外科手部消毒时长对减少手部常驻菌群的影响，用于消毒剂和防腐剂的实验及应用测试。
我们的方法依据奥地利外科手部消毒产品测试指南。对21名受试者一只手在消毒前和消毒后立即进行手部细菌菌群释放评估，另一只手在戴手套3小时后进行评估。计算平均减少因子（RF）。
使用正丙醇或异丙醇时，分别应用1、3或5分钟后的即时平均log(10) RF分别为1.05、2.03和2.30以及0.74、1.
