Bouffler S D, Finnon P, Blasco M A, Ainsbury E
Health Protection Agency, Radiation Protection Division, Chilton, Didcot, UK.
Cytogenet Genome Res. 2008;122(3-4):292-6. doi: 10.1159/000167815. Epub 2009 Jan 30.
Telomeres are specialised structures at the ends of mammalian chromosomes with many unique properties. Recombinational events at telomeres are more frequent than in the remainder of the genome by several orders of magnitude. This study examined the influence of telomerase status and telomere length on genome-wide recombination assessed by genomic sister chromatid exchange (G-SCE). Telomerase deficiency per se appears to increase G-SCE frequencies in splenocytes but as telomeres shorten through subsequent generations of mTerc(-/-) mice this increase is progressively lost. Telomerase status and telomere length also influences the induction of G-SCE by UV light. Even when mitotic recombination is affected by PARP deficiency, mTerc and telomere length interact to further affect G-SCE frequencies. Taken together the data presented here demonstrate that telomerase status and telomere length can affect recombination frequencies genome-wide.
端粒是哺乳动物染色体末端具有许多独特性质的特殊结构。端粒处的重组事件比基因组其余部分的重组事件频繁几个数量级。本研究通过基因组姐妹染色单体交换（G-SCE）评估了端粒酶状态和端粒长度对全基因组重组的影响。端粒酶缺乏本身似乎会增加脾细胞中的G-SCE频率，但随着端粒在后续几代mTerc(-/-)小鼠中缩短，这种增加会逐渐消失。端粒酶状态和端粒长度也会影响紫外线对G-SCE的诱导。即使有丝分裂重组受到PARP缺乏的影响，mTerc和端粒长度仍会相互作用，进一步影响G-SCE频率。综合此处呈现的数据表明，端粒酶状态和端粒长度可影响全基因组的重组频率。
