Xiao Liang, Xie Chi-Chu, Cai Jun, Lin Zhi-Jie, Chen Yue-Hua
Department of Microbiology, College of Life Sciences, Nankai University, Tianjin, PR China.
Curr Microbiol. 2009 May;58(5):528-33. doi: 10.1007/s00284-009-9363-5. Epub 2009 Feb 3.
Strain MY75 is a gram-positive, aerobic, endospore-forming bacterium that can secrete high levels of extracellular chitinase (4.645 U/ml) when chitin powder exists as an inducer. This strain was identified as Bacillus licheniformis using the Biolog MicroLog microbial identification system and sequence analysis of 16S rDNA, gyrA and rpoB genes. Strain MY75 has the ability to inhibit the growth of Gibberella saubinetii and Aspergillus niger, two major pathogenic fungi in agriculture, and to restrain their spore germination completely. The chitinase was proved to play an important role in the strain's antifungal activity.
菌株MY75是一种革兰氏阳性、需氧、形成芽孢的细菌，当以几丁质粉末作为诱导剂时，它能够分泌高水平的胞外几丁质酶（4.645 U/ml）。使用Biolog MicroLog微生物鉴定系统以及16S rDNA、gyrA和rpoB基因的序列分析，该菌株被鉴定为地衣芽孢杆菌。菌株MY75能够抑制农业中两种主要致病真菌——小麦赤霉和黑曲霉的生长，并能完全抑制它们的孢子萌发。已证明几丁质酶在该菌株的抗真菌活性中发挥重要作用。
