Cheng Xiaomao, Xu Jinsong, Xia Shu, Gu Jianxun, Yang Yuan, Fu Jie, Qian Xiaoju, Zhang Shunchang, Wu Jiangsheng, Liu Kede
National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan, 430070, China.
Theor Appl Genet. 2009 Apr;118(6):1121-31. doi: 10.1007/s00122-009-0967-8. Epub 2009 Feb 4.
Microsatellite or simple sequence repeat (SSR) markers are routinely used for tagging genes and assessing genetic diversity. In spite of their importance, there are limited numbers of SSR markers available for Brassica crops. A total of 627 new SSR markers (designated BnGMS) were developed based on publicly available genome survey sequences and used to survey polymorphisms among six B. napus cultivars that serve as parents for established populations. Among these SSR markers, 591 (94.3%) successfully amplified at least one fragment and 434 (73.4%) detected polymorphism among the six B. napus cultivars. No correlation was observed between SSR motifs, repeat number or repeat length with polymorphism levels. A linkage map was constructed using 163 newly developed BnGMS marker loci and anchored with 164 public SSRs in a doubled haploid population. These new markers are evenly distributed over all linkage groups (LGs). Given that the majority of these SSRs are derived from bacterial artificial chromosome (BAC) end sequences, they will be useful in the assignment of their cognate BACs to LGs and facilitate the integration of physical maps with genetic maps for genome sequencing in B. napus.
微卫星或简单序列重复（SSR）标记常用于基因标记和遗传多样性评估。尽管其很重要，但甘蓝型油菜作物可用的SSR标记数量有限。基于公开的基因组调查序列开发了总共627个新的SSR标记（命名为BnGMS），并用于检测六个用作既定群体亲本的甘蓝型油菜品种之间的多态性。在这些SSR标记中，591个（94.3%）成功扩增出至少一个片段，434个（73.4%）在六个甘蓝型油菜品种间检测到多态性。未观察到SSR基序、重复次数或重复长度与多态性水平之间存在相关性。利用163个新开发的BnGMS标记位点构建了一个连锁图谱，并在一个双单倍体群体中用164个公共SSR进行了锚定。这些新标记均匀分布在所有连锁群（LG）上。鉴于这些SSR大多来自细菌人工染色体（BAC）末端序列，它们将有助于将其同源BAC分配到LG，并促进甘蓝型油菜基因组测序中将物理图谱与遗传图谱整合。
