Xu Yu, Zhang Bingbing, Ma Ning, Liu Xia, Qin Mengfan, Zhang Yan, Wang Kai, Guo Na, Zuo Kaifeng, Liu Xiang, Zhang Miao, Huang Zhen, Xu Aixia
State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling, China.
Institute of Vegetables and Flowers, Jiangxi Academy of Agricultural Sciences, Nanchang, China.
Front Plant Sci. 2021 Feb 3;11:626205. doi: 10.3389/fpls.2020.626205. eCollection 2020.
Flowering time plays a vital role in determining the life-cycle period, yield, and seed quality of rapeseed ( L.) in certain environments. Quantitative trait locus (QTL) mapping to identify the genetic architecture of genes controlling flowering time helps accelerate the early maturity breeding process. In this study, simple sequence repeats (SSR) and specific-locus amplified fragment sequencing (SLAF-seq) technologies were adopted to map the QTLs for flowering time in four environments. As a result, three target intervals, , , and were identified. Among this, was considered as a novel interval, and as stable regions. Based on the parental re-sequencing data, 7,022 single nucleotide polymorphisms (SNPs) and 2,195 insertion-deletions (InDels) between the two parents were identified in these three target regions. A total of 186 genes possessed genetic variations in these intervals, 14 of which were related to flowering time involved in photoperiod, circadian clock, vernalization, and gibberellin pathways. Six InDel markers linked to flowering time were developed in the three target intervals, indicating that the results were credible in this study. These results laid a good foundation for further genetic studies on flowering-time regulation in L.
在某些环境中，开花时间对决定油菜（L.）的生命周期、产量和种子质量起着至关重要的作用。通过数量性状位点（QTL）定位来确定控制开花时间的基因的遗传结构，有助于加速早熟育种进程。在本研究中，采用简单序列重复（SSR）和特异性位点扩增片段测序（SLAF-seq）技术，在四种环境下对开花时间的QTL进行定位。结果，确定了三个目标区间， 、 和 。其中， 被认为是一个新的区间， 和 是稳定区域。基于亲本重测序数据，在这三个目标区域中鉴定出两亲本间7022个单核苷酸多态性（SNP）和2195个插入缺失（InDel）。共有186个基因在这些区间存在遗传变异，其中14个与开花时间相关，涉及光周期、生物钟、春化作用和赤霉素途径。在这三个目标区间开发了6个与开花时间连锁的InDel标记，表明本研究结果可靠。这些结果为进一步研究油菜开花时间调控的遗传机制奠定了良好基础。
