Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
J Appl Microbiol. 2009 Apr;106(4):1101-10. doi: 10.1111/j.1365-2672.2008.04073.x. Epub 2009 Jan 16.
Complexity involved in the transport of soils and the restrictive legislation for the area makes on-site bioremediation the strategy of choice to reduce hydrocarbons contamination in Antarctica. The effect of biostimulation (with N and P) and bioaugmentation (with two bacterial consortia and a mix of bacterial strains) was analysed by using microcosms set up on metal trays containing 2.5 kg of contaminated soil from Marambio Station. At the end of the assay (45 days), all biostimulated systems showed significant increases in total heterotrophic aerobic and hydrocarbon-degrading bacterial counts. However, no differences were detected between bioaugmented and nonbioaugmented systems, except for J13 system which seemed to exert a negative effect on the natural bacterial flora. Hydrocarbons removal efficiencies agreed with changes in bacterial counts reaching 86 and 81% in M10 (bioaugmented) and CC (biostimulated only) systems. Results confirmed the feasibility of the application of bioremediation strategies to reduce hydrocarbon contamination in Antarctic soils and showed that, when soils are chronically contaminated, biostimulation is the best option. Bioaugmentation with hydrocarbon-degrading bacteria at numbers comparable to the total heterotrophic aerobic counts showed by the natural microflora did not improve the process and showed that they would turn the procedure unnecessarily more complex.
土壤运输的复杂性以及该地区的限制性立法使得现场生物修复成为减少南极洲碳氢化合物污染的首选策略。通过使用金属托盘上的微宇宙来分析生物刺激（N 和 P）和生物增强（两种细菌群落和细菌菌株的混合物）的效果，微宇宙中包含了来自马尔万巴站的 2.5 公斤受污染土壤。在试验结束时（45 天），所有生物刺激系统的总需氧异养菌和碳氢化合物降解细菌计数均显著增加。然而，除了 J13 系统似乎对自然细菌菌群产生负面影响外，生物增强和非生物增强系统之间没有检测到差异。碳氢化合物去除效率与细菌计数的变化一致，在 M10（生物增强）和 CC（仅生物刺激）系统中达到了 86%和 81%。结果证实了应用生物修复策略减少南极洲土壤中碳氢化合物污染的可行性，并表明在土壤长期污染的情况下，生物刺激是最佳选择。以与自然微生物群落表现出的总需氧异养菌数量相当的数量进行碳氢化合物降解细菌的生物增强并没有改善该过程，并且表明这会使该过程不必要地更加复杂。
