Abdullah Kristopher, Wilkins Daniel, Ferrari Belinda C
Faculty of Science, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, Australia.
Environmental Stewardship Program, Australian Antarctic Division, Department of Climate Change, Energy, Environment and Water, Kingston, TAS, Australia.
Front Microbiol. 2023 Jun 16;14:1113102. doi: 10.3389/fmicb.2023.1113102. eCollection 2023.
Hydrocarbon spills in cold climates are a prominent and enduring form of anthropogenic contamination. Bioremediation is one of a suite of remediation tools that has emerged as a cost-effective strategy for transforming these contaminants in soil, ideally into less harmful products. However, little is understood about the molecular mechanisms driving these complex, microbially mediated processes. The emergence of - technologies has led to a revolution within the sphere of environmental microbiology allowing for the identification and study of so called 'unculturable' organisms. In the last decade, - technologies have emerged as a powerful tool in filling this gap in our knowledge on the interactions between these organisms and their environment . Here, we utilize the text mining software Vosviewer to process meta-data and visualize key trends relating to cold climate bioremediation projects. The results of text mining of the literature revealed a shift over time from optimizing bioremediation experiments on the macro/community level to, in more recent years focusing on individual organisms of interest, interactions within the microbiome and the investigation of novel metabolic degradation pathways. This shift in research focus was made possible in large part by the rise of studies allowing research to focus not only what organisms/metabolic pathways are present but those which are functional. However, all is not harmonious, as the development of downstream analytical methods and associated processing tools have outpaced sample preparation methods, especially when dealing with the unique challenges posed when analyzing soil-based samples.
寒冷气候下的碳氢化合物泄漏是一种突出且持久的人为污染形式。生物修复是一系列修复工具之一，已成为一种具有成本效益的策略，用于在土壤中转化这些污染物，理想情况下将其转化为危害较小的产物。然而，对于驱动这些复杂的、微生物介导过程的分子机制，我们了解甚少。[此处原文缺失相关技术名称]技术的出现引发了环境微生物学领域的一场革命，使得人们能够识别和研究所谓的“不可培养”生物。在过去十年中，[此处原文缺失相关技术名称]技术已成为填补我们在这些生物与其环境之间相互作用知识空白的有力工具。在此，我们利用文本挖掘软件Vosviewer来处理元数据，并可视化与寒冷气候生物修复项目相关的关键趋势。文献文本挖掘结果显示，随着时间的推移，研究重点从在宏观/群落层面优化生物修复实验，转变为近年来关注感兴趣的单个生物、微生物群落内部的相互作用以及新型代谢降解途径的研究。研究重点的这种转变在很大程度上得益于[此处原文缺失相关研究类型]研究的兴起，这类研究使研究不仅能够关注存在哪些生物/代谢途径，还能关注哪些是有功能的。然而，并非一切都很和谐，因为下游分析方法和相关处理工具的发展超过了样品制备方法，尤其是在处理分析土壤样品时所面临的独特挑战时。
