Chaiyarit Ponlatham, Jintakanon Darunee, Klanrit Poramaporn, Siritapetawee Mookhda, Thongprasom Kobkan
Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, Thailand.
J Oral Pathol Med. 2009 Jan;38(1):55-62. doi: 10.1111/j.1600-0714.2008.00713.x.
Survivin is an inhibitor of apoptosis protein. Heat shock protein 90 (Hsp90) is a molecular chaperone that is involved in protein folding and maturation. Alterations in the expression of survivin and Hsp90 have been reported in several chronic inflammatory diseases. No study has evaluated the expression of survivin and Hsp90 in the same lesion of oral lichen planus (OLP). The objective of this study was to investigate the expression of survivin and Hsp90 in biopsy specimens of patients with OLP and controls.
Formalin-fixed and paraffin-embedded biopsy specimens from 29 patients with OLP and 29 healthy subjects were used in this study. Immunohistochemical methods were used to detect the expression of survivin and Hsp90 in the oral epithelia of these two groups. The distributions of positively stained epithelial cells were evaluated with a semiquantitative method, using an immunostaining score system.
Expression of survivin and Hsp90 in oral epithelia of patients with OLP was significantly different in comparison with that in controls (P = 0.001 and P < 0.001 respectively). These differences were in the direction towards reduction in the expression of survivin and Hsp90 in the oral epithelia of patients with OLP.
Our results provided additional evidence that alterations in the expression of survivin and Hsp90 in oral epithelia might be associated with pathological stress, such as chronic inflammation, in patients with OLP.
生存素是一种凋亡抑制蛋白。热休克蛋白90（Hsp90）是一种分子伴侣，参与蛋白质折叠和成熟过程。在几种慢性炎症性疾病中，已有关于生存素和Hsp90表达改变的报道。尚无研究评估口腔扁平苔藓（OLP）同一病变中生存素和Hsp90的表达情况。本研究的目的是调查OLP患者活检标本及对照组中生存素和Hsp90的表达情况。
本研究使用了来自29例OLP患者和29名健康受试者的福尔马林固定、石蜡包埋活检标本。采用免疫组织化学方法检测这两组口腔上皮中生存素和Hsp90的表达。使用免疫染色评分系统，通过半定量方法评估阳性染色上皮细胞的分布。
与对照组相比，OLP患者口腔上皮中生存素和Hsp90的表达有显著差异（分别为P = 0.001和P < 0.001）。这些差异表现为OLP患者口腔上皮中生存素和Hsp90的表达降低。
我们的结果提供了额外的证据，表明OLP患者口腔上皮中生存素和Hsp90表达的改变可能与慢性炎症等病理应激有关。
