Department of Stomatology III, Faculty of Odontology, Complutense University of Madrid, Madrid, Spain.
Oral Dis. 2013 Jan;19(1):65-72. doi: 10.1111/j.1601-0825.2012.01951.x. Epub 2012 Jul 2.
Heat-shock protein 27 (hsp27) has been implicated in several biological events. In this experimental study, we aimed at analysing, for the first time, the expression of hsp27 in the diverse stages of oral lichen planus (OLP) lesions.
Thirty-six biopsy specimens of patients with OLP and 10 of healthy patients were selected. OLP specimens were divided into three groups: G1 - moderate or mildly active OLP; G2 - active or moderately active atrophic OLP; G3 - mild or inactive atrophic OLP. Hsp27 expression was analysed by immunohistochemistry (staining intensity and percentage of stained cells), and results of staining were compared between the different groups. Gender, age and anatomical location were also studied.
In the basal layer, an increase of hsp27 expression in both G2 and G3 was observed when compared to G1 and control group. In contrast, a decrease of hsp27 expression in the superficial layer was observed in all groups when compared to control group.
The increased expression of Hsp27 in the basal layer observed during the OLP evolution and the less staining in the superficial layers in all cases of OLP suggest that hsp27 may have a role in the OLP pathogenesis.
热休克蛋白 27（hsp27）参与了多种生物学事件。在这项实验研究中，我们首次旨在分析 hsp27 在口腔扁平苔藓（OLP）病变的不同阶段的表达。
选择 36 例 OLP 患者和 10 例健康患者的活检标本。OLP 标本分为三组：G1 - 中度或轻度活动 OLP；G2 - 活跃或中度萎缩性 OLP；G3 - 轻度或非活跃萎缩性 OLP。通过免疫组织化学（染色强度和染色细胞百分比）分析 hsp27 的表达，并比较不同组之间的染色结果。还研究了性别、年龄和解剖位置。
在基底细胞层中，与 G1 和对照组相比，G2 和 G3 中 hsp27 的表达增加。相比之下，与对照组相比，所有 OLP 组的浅层 hsp27 表达减少。
OLP 进展过程中观察到基底细胞层中 hsp27 的表达增加，而所有 OLP 病例的浅层染色减少，提示 hsp27 可能在 OLP 发病机制中起作用。
