Adachi Y, Kamisako T, Yamamoto T
Second Department of Internal Medicine, Kinki University School of Medicine, Osakasayama, Japan.
J Lab Clin Med. 1991 Sep;118(3):261-8.
To elucidate the possible implication of hepatic blood supply to the occurrence of hepatolithiasis, the rat superior mesenteric vein, which drains blood from the intestine, or the splenic vein, which drains from the spleen, was occluded for 30 minutes. Changes in the hepatic oxygen saturation index, intrahepatic uridine diphosphate-glucuronic acid concentration, bile flow, and the excretion of bilirubin as well as its fraction, along with bile acid in bile before and after the procedure, were observed. In association with superior mesenteric vein occlusion, oxygen saturation index, hepatic uridine diphosphate-glucuronic acid concentration, bile flow, bile acid concentration in bile, and percentage of biliary bilirubin diglucuronide were all decreased. Incubation of bile under sterile conditions from rats with occluded superior mesenteric veins resulted in precipitation of mainly calcium salt of fatty acid. In contrast, splenic vein occlusion caused no changes except for a decrease in biliary bilirubin concentration. Incubation of bile from rats with occluded splenic veins did not induce precipitation. From these findings it can be concluded that blood flow in the superior mesenteric vein is the primary source of oxygen supply to the rat liver and that this vein plays an important role in maintaining bile flow, bile acid excretion, and bilirubin conjugation and in preventing the precipitation of bile (possibly preventing hepatolithiasis).
为阐明肝脏血液供应对肝内胆管结石形成的可能影响，将大鼠的肠系膜上静脉（从肠道引流血液）或脾静脉（从脾脏引流血液）阻断30分钟。观察手术前后肝脏氧饱和度指数、肝内尿苷二磷酸 - 葡萄糖醛酸浓度、胆汁流量、胆红素及其组分的排泄以及胆汁中胆汁酸的变化。与肠系膜上静脉阻断相关，氧饱和度指数、肝内尿苷二磷酸 - 葡萄糖醛酸浓度、胆汁流量、胆汁中胆汁酸浓度以及胆汁中胆红素二葡萄糖醛酸酯的百分比均降低。对肠系膜上静脉阻断的大鼠胆汁进行无菌条件下孵育，主要导致脂肪酸钙盐沉淀。相比之下，脾静脉阻断除了胆汁胆红素浓度降低外没有引起其他变化。对脾静脉阻断的大鼠胆汁进行孵育未诱导沉淀。从这些发现可以得出结论，肠系膜上静脉的血流是大鼠肝脏氧气供应的主要来源，并且该静脉在维持胆汁流动、胆汁酸排泄、胆红素结合以及防止胆汁沉淀（可能预防肝内胆管结石）方面发挥重要作用。
