甲状腺激素调控心肌梗死后大鼠的基因表达。
Regulation of gene expression with thyroid hormone in rats with myocardial infarction.
机构信息
Department of Biomedical Sciences, New York College of Osteopathic Medicine of New York Institute of Technology, Old Westbury, New York, United States of America.
出版信息
PLoS One. 2012;7(8):e40161. doi: 10.1371/journal.pone.0040161. Epub 2012 Aug 1.
INTRODUCTION
The expression of hundreds of genes is altered in response to left ventricular (LV) remodeling following large transmural myocardial infarction (MI). Thyroid hormone (TH) improves LV remodeling and cardiac performance after MI. However, the molecular basis is unknown.
METHODS
MI was produced by ligation of the left anterior descending coronary artery in female SD rats. Rats were divided into the following groups: (1) Sham MI, (2) MI, and (3) MI+T4 treatment (T4 pellet 3.3 mg, 60 days release, implanted subcutaneously immediately following MI). Four weeks after surgery, total RNA was isolated from LV non-infarcted areas for microarray analysis using the Illumina RatRef-12 Expression BeadChip Platform.
RESULTS
Signals were detected in 13,188 genes (out of 22,523), of which the expression of 154 genes were decreased and the expression of 200 genes were increased in MI rats compared with Sham MI rats (false discovery rate (FDR) <0.05). Compared to MI rats, T4 treatment decreased expression of 27 genes and increased expression of 28 genes. In particular, 6 genes down-regulated by MI and 12 genes up-regulated by MI were reversed by T4. Most of the 55 genes altered by T4 treatment are in the category of molecular function under binding (24) and biological processes which includes immune system process (9), multi-organism process (5) and biological regulation (19) nonexclusively.
CONCLUSIONS
These results suggest that altered expression of genes for molecular function and biological process may be involved in the beneficial effects of thyroid hormone treatment following MI in rats.
简介
成百上千的基因表达会因左心室（LV）在大面积透壁性心肌梗死（MI）后的重塑而改变。甲状腺激素（TH）可改善 MI 后的 LV 重塑和心脏功能。然而，其分子基础尚不清楚。
方法
通过结扎雌性 SD 大鼠的左前降支冠状动脉来制造 MI。将大鼠分为以下几组：（1）Sham MI，（2）MI，和（3）MI+T4 治疗组（T4 微球 3.3mg，60 天释放，MI 后立即皮下植入）。手术后 4 周，从 LV 非梗死区分离总 RNA，使用 Illumina RatRef-12 Expression BeadChip 平台进行微阵列分析。
结果
在 22523 个基因中检测到了 13188 个信号，其中 MI 大鼠的 154 个基因表达下调，200 个基因表达上调，与 Sham MI 大鼠相比（假发现率（FDR）<0.05）。与 MI 大鼠相比，T4 治疗使 27 个基因的表达下调，28 个基因的表达上调。特别是，MI 下调的 6 个基因和 MI 上调的 12 个基因被 T4 逆转。T4 治疗改变的 55 个基因大多数是在结合（24）和包括免疫系统过程（9）、多器官过程（5）和生物调节（19）在内的生物过程中的分子功能类别下改变的，并不排他。
结论
这些结果表明，分子功能和生物过程相关基因的表达改变可能参与了甲状腺激素治疗大鼠 MI 后的有益作用。
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