与羊绒性状相关的山羊催乳素基因（PRL）新单核苷酸多态性

Novel SNP of the goat prolactin gene (PRL) associated with cashmere traits.

作者信息

Lan X-Y, Pan Ch-Y, Chen H, Lei Ch-Z, Li F-Y, Zhang H-Y, Ni Y-S

机构信息

College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi, PR China.

出版信息

J Appl Genet. 2009;50(1):51-4. doi: 10.1007/BF03195652.

DOI:10.1007/BF03195652

PMID:19193983

Abstract

Concentrations of the single-chain polypeptide hormone prolactin (PRL) are associated with wool or cashmere traits, and its seasonal changes may determine patterns of enzymatic activity and may affect cashmere fibre growth and moult. So, the PRL gene is a potential candidate gene for cashmere traits in marker-assisted selection (MAS). In this paper, we report a novel missense single-nucleotide polymorphism (SNP) within the goat PRL gene in 1367 individuals by PCR-SSCP (polymerase chain reaction with single-strand conformation polymorphism) analysis and DNA sequencing. The novel X76049:g.576C>A mutation is confirmed by Eco24I PCR-RFLP (restriction fragment length polymorphism) analysis and causes a missense codon (Pro176Thr). The frequencies of allele C varied from 0.79 to 0.93 in 9 analysed goat populations. C allele was correlated with higher fibre length (P=0.014).

摘要

单链多肽激素催乳素（PRL）的浓度与羊毛或羊绒性状相关，其季节性变化可能决定酶活性模式，并可能影响羊绒纤维生长和换毛。因此，PRL基因是标记辅助选择（MAS）中羊绒性状的潜在候选基因。在本文中，我们通过PCR-SSCP（聚合酶链反应-单链构象多态性）分析和DNA测序，在1367个个体中报道了山羊PRL基因内一个新的错义单核苷酸多态性（SNP）。通过Eco24I PCR-RFLP（限制性片段长度多态性）分析证实了新的X76049:g.576C>A突变，该突变导致一个错义密码子（Pro176Thr）。在9个分析的山羊群体中，等位基因C的频率从0.79到0.93不等。C等位基因与较高的纤维长度相关（P=0.014）。