催乳素受体基因5'上游区域的一个新型单核苷酸多态性与辽宁绒山羊的纤维性状相关。

A novel single-nucleotide polymorphism in the 5' upstream region of the prolactin receptor gene is associated with fiber traits in Liaoning cashmere goats.

作者信息

Zhou J P, Zhu X P, Zhang W, Qin F, Zhang S W, Jia Z H

机构信息

State Key Laboratory for Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, PR China.

出版信息

Genet Mol Res. 2011 Oct 13;10(4):2511-6. doi: 10.4238/2011.October.13.8.

DOI:10.4238/2011.October.13.8

PMID:22009863

Abstract

The most important traits of Chinese Liaoning cashmere goat fiber are fiber diameter, weight, and length. We looked for polymorphisms and their possible association with cashmere fiber traits in the 5' upstream region (5' UTR) of the prolactin receptor gene (PRLR), which encodes an anterior pituitary peptide hormone involved in different physiological activities; it is the principal endocrine regulator in pelage replacement in mammals. A novel single-nucleotide polymorphism (SNP) was found in the 5' UTR of PRLR by PCR-RFLP in an analysis of 590 goats. Two genotypes (CC and CT) were observed. The frequencies of allele C and T were 0.93 and 0.07, respectively. Association analysis revealed that the PRLR 5' UTR polymorphism (SNP5) was significantly associated with cashmere fiber weight and diameter. This novel SNP in hircine PRLR has potential as a molecular marker for cashmere fiber weight and diameter in Liaoning cashmere goats.

摘要

中国辽宁绒山羊纤维最重要的特性是纤维直径、重量和长度。我们在催乳素受体基因（PRLR）的5'上游区域（5'UTR）寻找多态性及其与羊绒纤维特性的可能关联，该基因编码一种参与不同生理活动的垂体前叶肽激素；它是哺乳动物毛发更替的主要内分泌调节因子。通过对590只山羊的PCR-RFLP分析，在PRLR的5'UTR中发现了一个新的单核苷酸多态性（SNP）。观察到两种基因型（CC和CT）。等位基因C和T的频率分别为0.93和0.07。关联分析表明，PRLR 5'UTR多态性（SNP5）与羊绒纤维重量和直径显著相关。山羊PRLR中的这个新SNP有潜力作为辽宁绒山羊羊绒纤维重量和直径的分子标记。