College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, No 22 Xinong Road, Yangling, 712100 Shaanxi, China.
Mol Biol Rep. 2011 Mar;38(3):2037-42. doi: 10.1007/s11033-010-0327-4. Epub 2010 Sep 26.
Adipocyte determination and differentiation-dependent factor 1/sterol regulatory element-binding protein-1c (ADD1/SREBP1c) is a major determinant of tissue differential lipogenic capacity in mammalian and avian species. The objectives of the present study were to focus on insertion-deletion polymorphism (indel) in the bovine ADD1/SREBP1c gene, and analyzing its effect on growth traits in a sample of 1035 cattle belonging to four Chinese cattle breeds. PCR-SSCP, DNA sequencing and agarose electrophoresis methods were used. The 778 bp PCR products of ADD1/SREBP1c gene exhibited three genotypes and two alleles were revealed: W and D. Frequencies of the W allele varied from 0.8651 to 1.000. The associations of the 84 bp indel mutation of ADD1/SREBP1c gene with growth traits of 265 Nanyang cows were analyzed. The animals with genotype WD had significantly higher birth weight, body weight, average daily gain than those with genotype WW at birth, 6-, 12-, 18-, and 24-month old (P < 0.05 or P < 0.01). These results suggest that the indel mutation of bovine ADD1/SREBP1c gene may influence the growth traits in cattle.
脂肪细胞决定和分化依赖性因子 1/固醇调节元件结合蛋白-1c（ADD1/SREBP1c）是哺乳动物和禽类组织不同脂肪生成能力的主要决定因素。本研究的目的是关注牛 ADD1/SREBP1c 基因中的插入-缺失多态性（indel），并分析其对四个中国牛品种的 1035 头牛样本生长性状的影响。使用 PCR-SSCP、DNA 测序和琼脂糖电泳方法。ADD1/SREBP1c 基因的 778 bp PCR 产物显示出三种基因型和两种等位基因：W 和 D。W 等位基因的频率从 0.8651 到 1.000 不等。分析了 ADD1/SREBP1c 基因 84bp indel 突变与 265 头南阳牛生长性状的关系。在出生时、6、12、18 和 24 月龄时，基因型 WD 的动物的初生重、体重和平均日增重均显著高于基因型 WW 的动物（P<0.05 或 P<0.01）。这些结果表明，牛 ADD1/SREBP1c 基因的 indel 突变可能影响牛的生长性状。
