恶臭假单胞菌YZ-26丙氨酸消旋酶的结晶及初步X射线衍射分析
Crystallization and preliminary X-ray diffraction analysis of alanine racemase from Pseudomonas putida YZ-26.
作者信息
Liu Junlin, Feng Lei, Shi Yawei, Feng Wei
机构信息
Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of the Ministry of Education, Shanxi University, Taiyuan, People's Republic of China.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Oct 1;68(Pt 10):1240-2. doi: 10.1107/S1744309112036974. Epub 2012 Sep 29.
Abstract
A recombinant form of alanine racemase (Alr) from Pseudomonas putida YZ-26 has been crystallized by the sitting-drop vapour diffusion method. X-ray diffraction data were collected to 2.4 Å resolution. The crystals belong to the space group C222(1), with unit-cell parameters a = 118.08, b = 141.86, c = 113.83 Å, and contain an Alr dimer in the asymmetric unit. The Matthews coefficient and the solvent content were calculated to be 2.8 Å(3) Da(-1) and approximately 50%, respectively.
摘要
采用坐滴气相扩散法对恶臭假单胞菌YZ-26的重组丙氨酸消旋酶（Alr）进行了结晶。收集到了分辨率为2.4 Å的X射线衍射数据。晶体属于空间群C222(1)，晶胞参数a = 118.08、b = 141.86、c = 113.83 Å，不对称单元中含有一个Alr二聚体。计算得到的马修斯系数和溶剂含量分别为2.8 Å(3) Da(-1)和约50%。
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