从三株申克孢子丝菌中分离的 SsCBF 抗原片段的比较血清学研究。
A comparative serological study of the SsCBF antigenic fraction isolated from three Sporothrix schenckii strains.
机构信息
Laboratório de Micologia Celular e Proteômica (LMCProt), Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Brazil.
出版信息
Med Mycol. 2009 Dec;47(8):874-8. doi: 10.3109/13693780802695520.
A previous work showed that an antigenic fraction of Sporothrix schenckii, SsCBF, was specifically recognized by IgG antibodies present in patients' clinical specimens. The objective of this investigation was to compare the reactivity of this antigen recovered from three S. schenckii strains. ROC curve analysis revealed a variation in the sensitivity and specificity of the antigen derived from each of the strains, with a higher AUC for strain 1099-18. Moreover, the presence of the main O-glycosidically-linked epitopes described in the SsCBF fraction was ascertained. A significant reduction in SsCBF reactivity of all the strains was observed after beta-elimination confirming the presence of O-glycan epitopes. The antigen isolated from strain 1099-18 proved to be a more accurate diagnostic tool for serodiagnosis of sporotrichosis.
先前的工作表明，申克孢子丝菌的一种抗原性部分 SsCBF 被存在于患者临床标本中的 IgG 抗体特异性识别。本研究的目的是比较从三种申克孢子丝菌菌株中回收的这种抗原的反应性。ROC 曲线分析显示，来自每个菌株的抗原的敏感性和特异性存在差异，菌株 1099-18 的 AUC 更高。此外，还确定了 SsCBF 部分中描述的主要 O-糖基连接表位的存在。所有菌株的 SsCBF 反应性显著降低，这证实了 O-聚糖表位的存在。从菌株 1099-18 中分离出的抗原被证明是孢子丝菌病血清学诊断的更准确的诊断工具。
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