Universidade Federal de São Paulo, Disciplina de Biologia Celular, São Paulo, Brazil.
Vet Microbiol. 2011 Jan 27;147(3-4):445-9. doi: 10.1016/j.vetmic.2010.07.007. Epub 2010 Jul 16.
The main objective of this study is to standardize an ELISA for the diagnosis of feline sporotrichosis. Sporothrix schenckii is the etiological agent of human and animal sporotrichosis. Cats may act as reservoirs for S. schenckii and can transmit the infection to humans by a bite or scratch. There are few methods for the serological diagnosis of fungal diseases in animals. In this paper, an ELISA test for the diagnosis of cat sporotrichosis is proposed, which detects S. schenckii-specific antibodies in feline sera. Two different kinds of antigens were used: "SsCBF", a specific molecule from S. schenckii that consists of a Con A-binding fraction derived from a peptido-rhamnomannan component of the cell wall, and a S. schenckii crude exoantigen preparation. The ELISA was developed, optimized, and evaluated using sera from 30 cats with proven sporotrichosis (by culture isolation); 22 sera from healthy feral cats from a zoonosis center were used as negative controls. SsCBF showed 90% sensitivity and 96% specificity in ELISA; while crude exoantigens demonstrated 96% sensitivity and 98% specificity. The ELISA assay described here would be a valuable screening tool for the detection of specific S. schenckii antibodies in cats with sporotrichosis. The assay is inexpensive, quick to perform, easy to interpret, and permits the diagnosis of feline sporotrichosis.
本研究的主要目的是标准化用于诊断猫孢子丝菌病的 ELISA。申克孢子丝菌是人类和动物孢子丝菌病的病原体。猫可能是申克孢子丝菌的储存宿主，并可通过咬伤或抓伤将感染传播给人类。目前，动物真菌病的血清学诊断方法较少。本文提出了一种用于诊断猫孢子丝菌病的 ELISA 检测方法，该方法可检测猫血清中的申克孢子丝菌特异性抗体。使用了两种不同的抗原：“SsCBF”，一种来自申克孢子丝菌的特异性分子，由细胞壁中糖肽-鼠李糖甘露聚糖成分衍生的 ConA 结合部分组成，以及一种申克孢子丝菌粗外抗原制剂。通过对 30 例经培养分离证实患有孢子丝菌病的猫（30 例）和 22 例来自动物传染病中心的健康野生猫的血清进行开发、优化和评估。在 ELISA 中，SsCBF 的敏感性为 90%，特异性为 96%；而粗外抗原的敏感性为 96%，特异性为 98%。这里描述的 ELISA 检测将成为检测孢子丝菌病猫中特异性申克孢子丝菌抗体的有价值的筛选工具。该检测方法价格低廉、操作快速、易于解释，并可诊断猫孢子丝菌病。
