Adelowo O O, Fagade O E
Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomoso, Nigeria.
Lett Appl Microbiol. 2009 Feb;48(2):167-72. doi: 10.1111/j.1472-765X.2008.02523.x.
Previous analysis of tet39 suggests it may be present in other bacterial species. Hence, we investigated the host range of tet39 among bacterial from a poultry waste polluted river in Southwestern Nigeria.
Thirteen resistant bacterial isolated from the water and sediment of the polluted river was investigated for the presence of tetracycline resistance genes tetA, tetB, tetC, tet39 and the transposon integrase gene of the Tn916/1545 family by PCR. While tetA, tetB, tetC and integrase genes cannot be detected in any of the organisms, tet39 was detected in eight of the tested organisms including three Gram-positive species. Sequence analysis showed the genes have high sequence identities (> or =99%) with tet39 of Acinetobacter sp. LUH5605, the first and only bacterial genus from which the gene has been reported to date. This is a novel observation.
This study shows that apart from Acinetobacter, tet39 is present in other bacterial species tested in this study.
This study adds to available information on the occurrence and distribution of tet39 among environmental bacteria and suggests that the gene has a broader host range than previously reported.
先前对tet39的分析表明它可能存在于其他细菌物种中。因此，我们调查了尼日利亚西南部一条受家禽粪便污染河流中的细菌中tet39的宿主范围。
通过聚合酶链反应（PCR）对从受污染河流的水和沉积物中分离出的13株耐药细菌进行四环素耐药基因tetA、tetB、tetC、tet39以及Tn916/1545家族转座子整合酶基因的检测。虽然在任何一种生物体中都未检测到tetA、tetB、tetC和整合酶基因，但在8株受试生物体中检测到了tet39，其中包括3株革兰氏阳性菌。序列分析表明，这些基因与不动杆菌属LUH5605的tet39具有高度的序列同一性（≥99%），LUH5605是迄今为止报道该基因的首个也是唯一的细菌属。这是一个新的发现。
本研究表明，除了不动杆菌属外，tet39还存在于本研究中测试的其他细菌物种中。
本研究增加了关于tet39在环境细菌中的发生和分布的现有信息，并表明该基因的宿主范围比先前报道的更广。
