Chambers A F, Denhardt G H, Wilson S M, Cairncross J G
Department of Oncology, University of Western Ontario, London, Canada.
J Neurooncol. 1991 Aug;11(1):43-8. doi: 10.1007/BF00166996.
To examine the relationship between the malignant behavior of rat glioma cells and expression of the differentiation antigen glial fibrillary acidic protein (GF), we assayed the tumorigenicity and metastatic ability of P635 and its GF+ or GF- clones. We injected P635 (GF+) and clone 45 (GF-) cells intramuscularly in nude mice. Both lines formed local tumors which metastasized to lungs with equal efficiency. We then injected these lines intravenously in nude mice. Both produced experimental metastases in lungs and other organs with equal efficiency. Finally we injected P635 and several GF+ and GF- clones into the chorioallantoic membrane veins of naturally immune-deficient chick embryos and measured cell growth in embryonic liver. We again found that all clones survived and grew equally well. P635 cells are capable of extracranial growth and metastasis, two important features of the malignant phenotype. We conclude that GF in P635 is a neutral marker with respect to tumorigenicity and metastatic ability.
为研究大鼠胶质瘤细胞的恶性行为与分化抗原胶质纤维酸性蛋白（GF）表达之间的关系，我们检测了P635及其GF+或GF-克隆的致瘤性和转移能力。我们将P635（GF+）和克隆45（GF-）细胞肌肉注射到裸鼠体内。这两种细胞系均形成了局部肿瘤，并以相同的效率转移至肺部。然后我们将这些细胞系静脉注射到裸鼠体内。两者在肺部和其他器官产生实验性转移的效率相同。最后，我们将P635以及几个GF+和GF-克隆注射到自然免疫缺陷鸡胚的绒毛尿囊膜静脉中，并测量胚胎肝脏中的细胞生长情况。我们再次发现所有克隆均存活且生长良好。P635细胞能够在颅外生长和转移，这是恶性表型的两个重要特征。我们得出结论，P635中的GF在致瘤性和转移能力方面是一个中性标志物。
