Kane Nicole, Kelly Rodney, Saunders Philippa T K, Critchley Hilary O D
Medical Research Council Human Reproductive Sciences Unit, Queen's Medical Research Institute, Edinburgh, United Kingdom.
Endocrinology. 2009 Jun;150(6):2882-8. doi: 10.1210/en.2008-1309. Epub 2009 Feb 5.
The endometrial lining of the human uterus contains a population of phenotypically distinct (CD56(bright), CD16(dim)), tissue-specific, natural killer [uterine natural killer (uNK)] cells that play a key role in the establishment of a successful pregnancy. An increase in the number of endometrial uNK cells occurs when the conceptus implants, and there is a further increase during the early stages of placentation. Here, we describe studies that have identified human chorionic gonadotrophin (hCG), a glycoprotein synthesized by the preimplantation conceptus, as a novel regulator of uNK cell proliferation. The impact of hCG on uNK cells was mediated via the mannose receptor (CD206) rather than by the classical hCG/LH receptor that was not expressed. The mannose receptor and hCG were colocalized on the surface of uNK cells, and proliferation did not occur if cells were incubated with deglycosylated hCG or intact hCG in the presence of excess d-Mannose. These novel observations provide new insight into the endocrine-immune dialogue that exists between the conceptus and immune cells within the receptive endometrium, and have implications for the role of uNK cell-trophoblast interactions and pregnancy outcome.
人类子宫内膜含有一群表型不同（CD56(bright)、CD16(dim)）、组织特异性的自然杀伤细胞[子宫自然杀伤细胞（uNK）]，它们在成功妊娠的建立过程中发挥关键作用。当孕体着床时，子宫内膜uNK细胞数量增加，在胎盘形成的早期阶段进一步增加。在此，我们描述了一些研究，这些研究已确定由着床前孕体合成的糖蛋白人绒毛膜促性腺激素（hCG）是uNK细胞增殖的一种新型调节因子。hCG对uNK细胞的影响是通过甘露糖受体（CD206）介导的，而不是通过未表达的经典hCG/LH受体。甘露糖受体和hCG共定位于uNK细胞表面，如果在存在过量d-甘露糖的情况下将细胞与去糖基化hCG或完整hCG一起孵育，则不会发生增殖。这些新发现为孕体与接受性子宫内膜内免疫细胞之间存在的内分泌-免疫对话提供了新的见解，并对uNK细胞-滋养层相互作用的作用和妊娠结局具有启示意义。
