Trundley Anita, Gardner Lucy, Northfield Jacquie, Chang Chiwen, Moffett Ashley
Department of Pathology, University of Cambridge, United Kingdom.
Methods Mol Med. 2006;122:109-22. doi: 10.1385/1-59259-989-3:109.
During human pregnancy, fetal placental cells known as trophoblast invade into the uterine mucosal lining, coming into contact with maternal cells including a specialized population of leukocytes. In order to understand the interaction of maternal cells with trophoblast, it is useful to be able to isolate the various cell types from the fetal-maternal interface so that they may be characterized and co-cultured in vitro. This chapter details the methods we use in our laboratory for enrichment and/or purification of trophoblast cells, decidual leukocytes, decidual natural killer cells, decidual stromal cells, and decidual glandular epithelial cells from human first-trimester tissue samples.
在人类妊娠期间，被称为滋养层的胎儿胎盘细胞侵入子宫黏膜内层，与包括特定白细胞群体在内的母体细胞接触。为了了解母体细胞与滋养层之间的相互作用，能够从胎儿 - 母体界面分离出各种细胞类型以便在体外对其进行表征和共培养是很有用的。本章详细介绍了我们实验室用于从人类孕早期组织样本中富集和/或纯化滋养层细胞、蜕膜白细胞、蜕膜自然杀伤细胞、蜕膜基质细胞和蜕膜腺上皮细胞的方法。
