Erk I, Delacroix H, Nicolas G, Ranck J L, Lepault J
Centre de Génétique Moléculaire, CNRS, Gif-sur-Yvette, France.
J Electron Microsc Tech. 1991 Aug;18(4):406-10. doi: 10.1002/jemt.1060180409.
We are using and developing cryo-electron microscopy of vitrified specimens. Our main interests concern the structure of muscle and muscular components. Micrographs which generally contain periodic features are analyzed by numerical image processing methods. To detect artifacts induced by the electron microscopy techniques, we correlate our results to those obtained by X-ray diffraction. In this paper, we describe our approach to the study of bulk specimens. Vitrification of such specimens is assessed by cryo-X-ray diffraction. Microscopy is done on cryo-substituted specimens.
我们正在使用并开发玻璃化标本的冷冻电子显微镜技术。我们主要关注肌肉和肌肉成分的结构。通常包含周期性特征的显微照片通过数值图像处理方法进行分析。为了检测由电子显微镜技术引起的伪像，我们将我们的结果与通过X射线衍射获得的结果进行关联。在本文中，我们描述了我们对大块标本的研究方法。此类标本的玻璃化通过冷冻X射线衍射进行评估。显微镜检查是在冷冻替代标本上进行的。
