淀粉样纤维的透射电子显微镜检查。

Transmission electron microscopy of amyloid fibrils.

作者信息

Gras Sally L, Waddington Lynne J, Goldie Kenneth N

机构信息

Department of Chemical and Biomolecular Engineering, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia.

出版信息

Methods Mol Biol. 2011;752:197-214. doi: 10.1007/978-1-60327-223-0_13.

DOI:10.1007/978-1-60327-223-0_13

PMID:21713639

Abstract

Transmission Electron Microscopy of negatively stained and cryo-prepared specimens allows amyloid fibrils to be visualised at high resolution in a dried or a hydrated state, and is an essential method for characterising the morphology of fibrils and pre-fibrillar species. We outline the key steps involved in the preparation and observation of samples using negative staining and cryo-electron preservation. We also discuss methods to measure fibril characteristics, such as fibril width, from electron micrographs.

摘要

对经负染色和冷冻制备的标本进行透射电子显微镜观察，可在干燥或水合状态下以高分辨率观察淀粉样纤维，这是表征纤维和纤维前体形态的重要方法。我们概述了使用负染色和冷冻电子保存制备和观察样品所涉及的关键步骤。我们还讨论了从电子显微照片测量纤维特征（如纤维宽度）的方法。