Okamoto Cinthya Kimori, Gonçalves-De-Andrade Rute M, Queiroz Giselle Pidde, Gutierez Vanessa P, De Almeida Daniel Manzoni, Cury Yara, Bertani Rogério, Portaro Fernanda C V, Tambourgi Denise V
Laboratório de Imunoquímica, Instituto Butantan, Av. Prof. Vital Brazil, 1500, CEP 05503-900, São Paulo, Brazil.
J Med Entomol. 2009 Jan;46(1):58-66. doi: 10.1603/033.046.0108.
Ctenus medius Keyserling, 1891 (Araneae: Ctenidae) co-occurs in various microhabitats of the Brazilian Atlantic Forest and can be easily misidentified as the medically important spider Phoneutria nigriventer Keyserling, 1981 (Ctenidae). Despite being phylogenetically close to Phoneutria, no data are available about the toxic potential of Ctenus medius venom. Here we show that, although presenting different profile of protein composition, C. medius venom displays some of the toxic properties exhibited by P. nigriventer venom, including proteolytic, hyaluronidasic and phospholipasic activities, as well as the ability of causing hyperalgesia and edema. Moreover, C. medius venom interferes in the activation of the complement system in concentrations that P. nigriventer venom is inactive. Thus, these data show that venoms of spiders from Ctenidae family share important proinflammatory properties and suggest that the C. medius bite may have an important noxious effect in human accidents.
1891年的中型栉足蛛（Ctenus medius Keyserling，蜘蛛目：栉足蛛科）与巴西大西洋森林的各种微生境共生，并且很容易被误认作具有医学重要性的蜘蛛——1981年的黑腹盗蛛（Phoneutria nigriventer Keyserling，栉足蛛科）。尽管在系统发育上与盗蛛属接近，但关于中型栉足蛛毒液的毒性潜力尚无数据。在此我们表明，尽管中型栉足蛛毒液呈现出不同的蛋白质组成概况，但它具有一些黑腹盗蛛毒液所展现出的毒性特性，包括蛋白水解、透明质酸酶和磷脂酶活性，以及引起痛觉过敏和水肿的能力。此外，中型栉足蛛毒液在黑腹盗蛛毒液无活性的浓度下会干扰补体系统的激活。因此，这些数据表明栉足蛛科蜘蛛的毒液具有重要的促炎特性，并表明中型栉足蛛叮咬在人类意外事件中可能具有重要的有害影响。
